Lactobacillus plantarum TWK10 (LP10) is a probiotic known to improve endurance exercise performance. Here, we analyze the proteomics and metagenomic changes in a LP10 supplemented mouse model. Male ICR mice were divided into two groups (n = 8) to receive by oral gavage either vehicle or of LP10 for 6 weeks. Proteins changes by LP10 treatment were subjected to the Ingenuity Pathway Analysis (IPA) to provide corroborative evidence for differential regulation of molecular and cellular functions affecting metabolic processes. Fecal samples were obtained from each mouse, and the microbial community profile analyzed by pyrosequencing of the 16S rRNA genes. Of the 880 identified proteins, 25 proteins were significantly downregulated and 44 proteins were significantly upregulated in the LP10 treated compared to vehicle group. LP10 supplementation shift in the gut microbiota to butyrate‐producing members and provided from lipid oxidation since peroxisomal fatty acid oxidation in liver.

中文翻译：

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蛋白质组和微生物群分析强调补充植物乳杆菌 TWK10 可改善小鼠的能量代谢和运动表现。


植物乳杆菌TWK10 (LP10) 是一种益生菌，已知可以提高耐力运动表现。在这里，我们分析了补充 LP10 的小鼠模型中的蛋白质组学和宏基因组变化。雄性 ICR 小鼠被分为两组 ( n = 8)，通过口服强饲法接受赋形剂或 LP10，为期 6 周。对 LP10 处理引起的蛋白质变化进行了 Ingenuity Pathway Analysis (IPA)，为影响代谢过程的分子和细胞功能的差异调节提供确凿的证据。从每只小鼠身上获取粪便样本，并通过 16S rRNA 基因焦磷酸测序分析微生物群落概况。与赋形剂组相比，在 LP10 处理的 880 种已鉴定蛋白质中，25 种蛋白质显着下调，44 种蛋白质显着上调。 LP10 的补充使肠道微生物群转向产生丁酸盐的成员，并由肝脏中过氧化物酶体脂肪酸氧化产生的脂质氧化提供。