Oplopanax horridus, widely distributed in North America, is an herbal medicine traditionally used by Pacific indigenous peoples for various medical conditions. After oral ingestion, constituents in O. horridus extract (OhE) could be converted to their metabolites by the enteric microbiome before absorption. In this study, in order to mimic gut environment, the OhE was biotransformed using the enteric microbiome of healthy human subjects. For accurate and reliable data collection with optimized approaches in sample preparation and analytical conditions, ultra‐performance liquid chromatography and quadrupole time‐of‐flight mass spectrometry were used to characterize parent constituents and their metabolites. In the extract, 20 parent compounds were identified including polyynes, sesquiterpenes, monoterpeondids, phenylpropanoids and phenolic acids. After the biotransformation, a total of 78 metabolites were identified, of which 37 belonged to polyynes metabolites. The common biotransformation pathways are hydroxylation, acetylization, methylation and demethylation. Based on the pathway distributions, the metabolism signature of OhE has been explored. The metabolism pathways of OhE compounds are dependent on their structural classifications and hydrophilic/hydrophobic properties. In summary, with comprehensive analysis, we systematically investigated human microbiome‐derived OhE metabolites. The enteric microbial metabolism signature provides novel information for future effective use of O. horridus.

中文翻译：

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人肠微生物群来自北美本地植物horopusus Horridus的代谢特征，采用UPLC / Q-TOF-MS分析。

Oplopanax horridus在北美广泛分布，是太平洋土著人民传统上用于各种医疗条件的草药。口服摄入后，hor.us中的成分提取物（OhE）可以在吸收前被肠道微生物组转化为代谢产物。在这项研究中，为了模拟肠道环境，使用了健康人类受试者的肠道微生物组对OhE进行了生物转化。为了通过优化的样品制备和分析条件方法来准确，可靠地收集数据，超高效液相色谱和四极杆飞行时间质谱用于表征母体成分及其代谢产物。在提取物中，鉴定出20种母体化合物，包括聚炔，倍半萜，单萜，苯基丙烷和酚酸。生物转化后，共鉴定出78种代谢产物，其中37种属于多炔类代谢产物。常见的生物转化途径是羟基化，乙酰化，甲基化和脱甲基。基于途径分布，已经研究了OhE的代谢特征。OhE化合物的代谢途径取决于其结构分类和亲水/疏水特性。总之，通过全面的分析，我们系统地研究了人类微生物组衍生的OhE代谢产物。肠道微生物的新陈代谢特征为将来的有效使用提供了新的信息。O. horridus。