Until recently, the mammalian ovary was considered to consist of fully differentiated tissues, but evidence for the presence of adult stem cells in this organ appeared. The differentiation potential of these cells, referred to as putative stem cells, is not well defined. Porcine ovarian putative stem cells (poPSCs) were immunomagnetically isolated from postnatal pig ovaries based on the presence of the SSEA-4 surface marker protein. First, they were cultured in the undifferentiated state. After the third passage, a novel 7-day culture method inducing their differentiation into neural-like cells by the addition of forskolin (FSK), retinoic acid (RA) and basic fibroblast growth factor (bFGF) to the culture medium was applied. After 7 days, poPSCs successfully differentiated into neural-like cells, as evidenced by neural morphology and the presence of the neuronal markers nestin, NeuN, and GFAP, as confirmed by immunofluorescence, western blot, and real-time PCR. Electrophysiological analysis of potassium and sodium channel activity (patch clamp) confirmed that they indeed differentiated into neurons. The plasticity of poPSCs offers an excellent opportunity, especially in the field of neuroscience, since they can differentiate into neurons or glial cells. Although poPSCs might not be pluripotent cells, they also escape the rigid classification framework of adult stem cells.

中文翻译：

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从猪卵巢推定干细胞中高效生成神经样细胞——形态学表征和电生理学特性评估

直到最近，哺乳动物的卵巢还被认为是由完全分化的组织组成，但出现了该器官中存在成体干细胞的证据。这些被称为推定干细胞的细胞的分化潜能尚未明确定义。基于 SSEA-4 表面标记蛋白的存在，猪卵巢推定干细胞 (poPSC) 从出生后的猪卵巢中免疫磁性分离。首先，它们是在未分化状态下培养的。在第三次传代后，应用了一种新的 7 天培养方法，通过向培养基中添加毛喉素 (FSK)、视黄酸 (RA) 和碱性成纤维细胞生长因子 (bFGF)，诱导它们分化为神经样细胞。7天后，poPSCs成功分化为神经样细胞，神经形态学和神经元标记物巢蛋白、NeuN 和 GFAP 的存在证明了这一点，正如免疫荧光、蛋白质印迹和实时 PCR 所证实的那样。钾和钠通道活性（膜片钳）的电生理分析证实它们确实分化为神经元。poPSC 的可塑性提供了极好的机会，尤其是在神经科学领域，因为它们可以分化为神经元或神经胶质细胞。尽管 poPSC 可能不是多能细胞，但它们也摆脱了成体干细胞严格的分类框架。poPSC 的可塑性提供了极好的机会，尤其是在神经科学领域，因为它们可以分化为神经元或神经胶质细胞。尽管 poPSC 可能不是多能细胞，但它们也摆脱了成体干细胞严格的分类框架。poPSC 的可塑性提供了极好的机会，尤其是在神经科学领域，因为它们可以分化为神经元或神经胶质细胞。尽管 poPSC 可能不是多能细胞，但它们也摆脱了成体干细胞严格的分类框架。