Human pluripotent stem cells (PSCs) through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryopreserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine.

通过体细胞核移植（SCNT）的人多能干细胞（PSC）可能是再生医学的重要来源。干细胞的衍生效率低和人类卵母细胞的可及性是其应用的主要障碍。我们先前曾报道过H3K9me3脱甲基酶的过表达提高了SCNT的效率。在这里，我们对PSC系应用了一种改进的派生方法，首先获得了人类SCNT-PSC系，该人SCNT-PSC系来自捐赠的冻存卵母细胞和具有纯合人类白细胞抗原（HLA）类型的脐带血细胞。SCNT-PSC与胚胎干细胞（ESC）具有非常相似的特性，并且在体外和体内均显示出免疫相容性具有匹配HLA类型的人性化鼠标。我们的研究表明，使用捐赠的冻存卵母细胞和具有已知HLA类型的脐带血细胞的SCNT技术为建立人类HLA匹配的SCNT-PSC库作为再生医学提供了一种有希望的方法。