Cu²⁺ is an eminent trace metal ion in the human body as a structural cofactor that can enhance immune function. Aluminum is one of the most abundant elements found in the earth's crust and most biological tissues appear in Al³⁺ form. Hg²⁺ is probable to cause cancer with high toxicity. However, excess accumulation of Al³⁺, Cu²⁺ or Hg²⁺ in the body leads to many diseases. Therefore, detecting the level of Al³⁺, Cu²⁺ and Hg²⁺ are very important for human health. However, preparing a suitable single probe for the simultaneous detection of Al³⁺, Cu²⁺ and Hg²⁺ remains a challenge. TPE-based carbohydrazide derivative (TPE-Ch) was synthesized by the one-pot reaction. The TPE-Ch displayed notable AIE and AIEE phenomena and can act as a fluorescence sensor that turns on toward Al³⁺ in ethanol/water mixtures (f_w = 70%) and turns off toward Cu²⁺ or Hg²⁺. TPE-Ch can produce various assemblies by coordination with Al³⁺, Cu²⁺ and Hg²⁺, including nanospheres, tree-like assembly morphology and belt-like superstructures. Optical microscopy (OM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and fluorescence spectrography were employed to characterize the self-assemblies.

Cu ²⁺是人体中重要的微量金属离子，可作为增强免疫功能的结构辅因子。铝是在地壳中发现的最丰富的元素之一，大多数生物组织都以Al ³⁺形式出现。Hg ²⁺可能引起高毒性的癌症。然而，体内Al ³⁺，Cu ²⁺或Hg ^2+的过量积累会导致许多疾病。因此，检测Al ³⁺，Cu ²⁺和Hg ^2+的含量对人体健康非常重要。但是，准备适合同时检测Al ³⁺，Cu ²⁺和Hg的单个探针²⁺仍然是一个挑战。通过一锅法合成基于TPE的碳酰肼衍生物（TPE-Ch）。TPE-Ch显示出显着的AIE和AIEE现象，并且可以充当荧光传感器，该传感器在乙醇/水混合物（f _w  = 70％）中向Al ³⁺方向打开，而向Cu ²⁺或Hg ²⁺方向关闭。TPE-Ch可通过与Al ³⁺，Cu ²⁺和Hg ²⁺配合产生各种组装体，包括纳米球，树状组装体形态和带状超结构。使用光学显微镜（OM），扫描电子显微镜（SEM），X射线衍射（XRD）和荧光光谱来表征自组装体。