Actinobacteria are the major source of bioactive secondary metabolites and are featured in the search for antimicrobial compounds. We used nuclear magnetic resonance (RMN)-metabolic profiling and multivariate data analysis (MVDA) to correlate the metabolites’ production of Streptomyces sp. PNM-9 from the algae Dictyota sp. and their biological activity against the rice phytopathogenic bacteria Burkholderia spp. The compounds 2-methyl-N-(2′-phenylethyl)-butanamide (1) and 3-methyl-N-(2′-phenylethyl)-butanamide (2) were identified through MVDA and 2D NMR experiments in the organic extract of a 15-days LB media culture of Streptomyces sp. PNM-9. Compounds 1 and 2 were isolated and their structures confirmed by one- and two-dimensional NMR and mass spectrometry (MS) data. Compounds 1 and 2 were active against the rice pathogenic bacteria Burkholderia glumae (ATCC 33,617) displaying minimal inhibitory concentration (MIC) values of 2.43 mM and 1.21 mM, respectively. The metabolomics-guided approach employing NMR-metabolic profiling was useful for marine microbial bioprospecting and suggested Streptomyces sp. PNM-9 strain and its compounds as a potential control against phytopathogenic bacteria.

放线菌是生物活性次生代谢产物的主要来源，在寻找抗菌化合物中具有特色。我们使用核磁共振（RMN）-代谢谱和多元数据分析（MVDA）来关联链霉菌sp的代谢产物的产生。来自藻类Dictyota sp。的PNM-9 。及其对水稻植物致病菌伯克霍尔德氏菌的生物学活性。通过MVDA和2D NMR实验在有机提取物中鉴定了化合物2-甲基-N-（2'-苯乙基）丁酰胺（1）和3-甲基-N-（2'-苯乙基）丁酰胺（2）。链霉菌15天的LB培养基培养。PNM-9。化合物1分离出1和2，并通过一维和二维NMR和质谱（MS）数据确认其结构。化合物1和2对水稻致病细菌小球藻伯克霍尔德菌（ATCC 33,617）具有活性，分别显示最小抑菌浓度（MIC）值为2.43 mM和1.21 mM。代谢组学指导的采用NMR代谢谱分析的方法可用于海洋微生物生物勘探，并建议使用链霉菌。PNM-9菌株及其化合物可作为对抗植物病原菌的潜在控制。