The effect of two commercial formulations (incorporating mepanipyrim and tetraconazole as active substances) on the metabolism of Saccharomyces cerevisiae Lalvin T73™, growing on a synthetic grape must, and their influence on the alcoholic fermentation course and the biosynthesis of volatiles derived from phenylalanine catabolism was studied. No relevant effects were observed for mepanipyrim except for glycerol production. On the contrary, in the presence of tetraconazole many genes and some proteins related to cell cycle progression and mitosis were repressed. This fact could explain the lower biomass concentration and the lower sugar consumption registered for tetraconazole at the end of the study. However, the biomass-to-ethanol yield was higher in connection with the overexpression of the ADH1 gene. The presence of tetraconazole residues seems to accelerate the Ehrlich pathway. These results agree with the overexpression of several genes (BAT1, PDC1, PDC5, ADH1, SFA1, ATF2, PFK1, PFK2 and ARO3) and a higher abundance of two proteins (Gap1p and Atf2p) involved in this metabolic pathway.

两种商业配方（掺入潘尼吡喃和四康唑作为活性物质）对在合成葡萄汁上生长的酿酒酵母e Lalvin T73™代谢的影响，以及它们对酒精发酵过程和苯丙氨酸分解代谢衍生挥发物的生物合成的影响被研究了。除产生甘油外，未观察到甲氨y啶的相关影响。相反，在存在四康唑的情况下，许多与细胞周期进程和有丝分裂有关的基因和一些蛋白质受到抑制。这个事实可以解释研究结束时四康唑的较低生物量浓度和较低的糖消耗。但是，与ADH1的过表达有关，生物质转化为乙醇的产率更高基因。四康唑残基的存在似乎加速了Ehrlich途径。这些结果与几个基因（过表达BAT1，PDC1，PDC5，ADH1，SFA1，ATF2，PFK1，PFK2和ARO3）和较高的丰度参与该代谢途径的两种蛋白质（Gap1p和Atf2p）的。