In this work, a unique lysosome-targeted BODIPY photosensitizer named BOP-Lyso was designed for photodynamic therapy of cancer cells. The absorption wavelength of BOP-Lyso (10 μM) was located at 650 nm and its molar absorption coefficient was 8.2 × 10⁴ cm⁻¹M⁻¹. The emission wavelength of BOP-Lyso was at 709 nm. Photosensitizer BOP-Lyso showed excellent ability to produce singlet oxygen and the singlet oxygen yield of photosensitizer BOP-Lyso was calculated to be 43.1% using methylene blue as the reference with near-infrared led light irradiation (660 nm). From MTT (a common method for determining cell survival rate, MTT: 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide) assay, BOP-Lyso had extremely low dark toxicity (cell viability > 85%) and high photo toxicity. And the IC₅₀ value of BOP-Lyso was 0.24 μM, which was much lower than the previously reported literature. Besides that, DCFH-DA (2′,7′-Dichlorodihydrofluorescein diacetate) was employed to capture the generation of reactive oxygen species (ROS) in PDT process. Lysosomal colocalization experiments demonstrated that photosensitizer BOP-Lyso had good co-localization capability and the colocalization coefficient was calculated to be 0.92. Furthermore, photosensitizer BOP-Lyso had also been successfully applied to AO/EB cell staining experiments and exhibited excellent results. Moreover, cancer cells (SGC-7901 cells, human gastric carcinoma cells) migration status could be effectively inhibited after NIR led light irradiation with BOP-Lyso. On the whole, taking the above series of data into account, photosensitizer BOP-Lyso would play an important role in actual PDT of cancer cells.

在这项工作中，设计了一种名为BOP-Lyso的独特的靶向溶酶体的BODIPY光敏剂，用于癌细胞的光动力治疗。BOP-Lyso的吸收波长（10μM）位于650 nm，其摩尔吸收系数为8.2×10 ⁴  cm ^-1 M ^-1。BOP-Lyso的发射波长为709nm。光敏剂BOP-Lyso表现出优异的产生单线态氧的能力，使用亚甲基蓝作为参比，在近红外led光照射下（660 nm），光敏剂BOP-Lyso的单线态氧收率经计算为43.1％。通过MTT（一种确定细胞存活率的常用方法，MTT：3-（4，5-二甲基-2-噻唑基）-2，5-二苯基-2-H-四唑溴化物）测定，BOP-Lyso的暗度极低毒性（细胞活力> 85％）和高光毒性。和IC ₅₀BOP-Lyso的值为0.24μM，远低于先前报道的文献。除此之外，DCFH-DA（2'，7'-二氯二氢荧光素二乙酸酯）被用来捕获PDT过程中活性氧（ROS）的产生。溶酶体共定位实验表明，光敏剂BOP-Lyso具有良好的共定位能力，计算的共定位系数为0.92。此外，光敏剂BOP-Lyso也已成功应用于AO / EB细胞染色实验，并显示出优异的效果。此外，在NIR引导下用BOP-Lyso照射后，可以有效抑制癌细胞（SGC-7901细胞，人胃癌细胞）的迁移状态。总体而言，考虑到上述一系列数据，