Inefficient sewage management has caused contamination of our water resources (rivers and lakes) that inspires researchers to constantly innovate and upgrade the existing wastewater treatment technologies. This study explores the possibility of a microbial consortium immobilized on different natural matrix for sewage treatment at lab-scale bioreactor. Bacterial isolates having lipolytic, proteolytic, cellulolytic and amylolytic activities were included in consortia as these are the major constitutes of sewage. The activities of these bacterial cultures were confirmed using different microbiological and molecular tools. The microbial consortium immobilized on natural coconut coir was found to be the most effective matrix to enhance the efficiency of sewage treatment. The immobilization of microbes on a natural dry coconut coir matrix was confirmed through scanning electron microscopy (SEM). Two Lab-scale aerobic bioreactors were setup in parallel for the treatment of sewage and simulated wastewaters. Both the reactors were operated in continuous mode for 15 and 16 days and efficiency of treatment was analysed by monitoring chemical oxygen demand (COD) removal, nitrogen removal as NH 4 -N and NO 3 -N. Simultaneously, the nitrogen removal activity was validated in all members of microbial consortium using gene-specific primers. Results show that 75.9% and 73.7% COD removal were observed in column C and D and nitrogen as NO 3 -N 78.9% and 79.7% (NH 4 -N) removal were measured in the effluent of reactors. Similarly, 93% COD, 78.1% NO 3 -N and 79.7% NH 4 -N removal were observed in a stimulated wastewater reactor. This significant removal of organic material shows the higher efficiency of the reactor which could be used for field application for sewage treatment. The study proposes use of coconut coir as a suitable matrix for microbial immobilization and its application in treatment of domestic wastewater. The coconut coir matrix serves as a cost effective material for immobilization of lab designed consortium. Procedure was found to be an effective alternative for bioremediation.

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微生物群落固定在天然基质上用于废水生物修复

低效的污水管理已导致我们的水资源（河流和湖泊）受到污染，这促使研究人员不断创新和升级现有的污水处理技术。本研究探讨了固定在不同天然基质上的微生物聚生体在实验室规模的生物反应器中进行污水处理的可能性。具有脂肪分解、蛋白水解、纤维素分解和淀粉分解活性的细菌分离物被包括在聚生体中，因为这些是污水的主要成分。使用不同的微生物和分子工具证实了这些细菌培养物的活性。发现固定在天然椰子纤维上的微生物群落是提高污水处理效率的最有效基质。通过扫描电子显微镜 (SEM) 证实了微生物固定在天然干椰子椰壳基质上。两个实验室规模的好氧生物反应器平行设置用于处理污水和模拟废水。两个反应器均以连续模式运行 15 天和 16 天，并通过监测化学需氧量 (COD) 去除、NH 4 -N 和 NO 3 -N 的氮去除来分析处理效率。同时，使用基因特异性引物在微生物群落的所有成员中验证了脱氮活性。结果表明，在 C 和 D 柱中观察到 75.9% 和 73.7% 的 COD 去除率和氮，因为在反应器的流出物中测量到 NO 3 -N 78.9% 和 79.7% (NH 4 -N) 去除率。类似地，在模拟废水反应器中观察到 93% COD、78.1% NO 3 -N 和 79.7% NH 4 -N 去除率。有机物质的这种显着去除表明反应器的效率更高，可用于污水处理的现场应用。该研究建议使用椰子纤维作为微生物固定的合适基质及其在处理生活废水中的应用。椰子椰壳基质作为一种具有成本效益的材料，用于固定实验室设计的财团。发现程序是生物修复的有效替代方法。椰子椰壳基质作为一种具有成本效益的材料，用于固定实验室设计的财团。发现程序是生物修复的有效替代方法。椰子椰壳基质作为一种具有成本效益的材料，用于固定实验室设计的财团。发现程序是生物修复的有效替代方法。