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Automated in-process characterization and selection of cell-clones for quality and efficient cell manufacturing.
Cytotechnology ( IF 2.2 ) Pub Date : 2020-06-04 , DOI: 10.1007/s10616-020-00403-w
Venkata P Mantripragada 1 , Viviane Luangphakdy 1 , Bradley Hittle 2 , Kimerly Powell 2 , George F Muschler 1, 3
Affiliation  

Delivery of safe, effective and reliable cellular therapies, whether based on mesenchymal stromal cells (MSCs) or induced pluripotent stem cells (iPSCs), demand standardization of cell culture protocols. There is a need to develop automation platform that enables the users to generate culture expanded human cell populations that improves the quality and reduces batch-to-batch variation with respect to biological potential. Cell X™ robot was designed to address these current challenges in the cell fabrication industry. It utilizes non-invasive large field of view quantitative image analysis to guide an automated process of targeted “biopsy” (cells or media), “picking” (selection) of desired cells or colonies, or “weeding” (removal) of undesired cells, thus providing an unprecedented ability to acquire quantitative measurement in a complex heterogeneous cell environment “in process” and then to act on those measurements to define highly reproducible methods for cell and colony “management” based on application specific critical quality attributes to improve the quality of the manufactured cell lines and cell products.



中文翻译:

自动化过程中表征和细胞克隆选择,以实现高质量和高效的细胞制造。

提供安全、有效和可靠的细胞疗法,无论是基于间充质基质细胞 (MSC) 还是诱导多能干细胞 (iPSC),都需要细胞培养方案的标准化。需要开发自动化平台,使用户能够生成培养扩展的人类细胞群,从而提高质量并减少批次间在生物学潜力方面的差异。Cell X™ 机器人旨在解决当前电池制造行业的这些挑战。它利用非侵入性大视野定量图像分析来指导靶向“活检”(细胞或培养基)、“挑选”(选择)所需细胞或集落或“除草”(去除)不需要的细胞的自动化过程,

更新日期:2020-06-04
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