In this work, we developed a triple-parameter strategy for the detection of telomerase activity from cancer cells and urine samples. This strategy was developed based on magnetic bead-enzyme hybrids combined with fluorescence analysis, colorimetric assay, or adenosine triphosphate (ATP) meter as readout. The application of magnetic bead-enzyme hybrids has the advantages of magnetic separation and signal amplification. These detection methods can be used individually or in combination to achieve the optimal sensing performance and make the results more convincing. Among them, the ATP meter with portable size had easy operation and low cost, and this response strategy provided a higher sensitivity at the single-cell level. The designed strategy was suitable as naked-eye sensor and point-of-care testing (POCT) for rapid assaying of telomerase activity.

Magnetic bead-enzyme assemble for triple-parameter telomerase detection

在这项工作中，我们开发了一种从癌细胞和尿液样本中检测端粒酶活性的三参数策略。该策略是基于磁珠-酶杂交体与荧光分析，比色测定或三磷酸腺苷（ATP）仪相结合的读数而开发的。磁珠-酶杂交体的应用具有磁分离和信号放大的优点。这些检测方法可以单独使用，也可以组合使用，以实现最佳的传感性能，并使结果更具说服力。其中，具有便携式尺寸的ATP测量仪易于操作且成本低廉，并且这种响应策略在单细胞水平上提供了更高的灵敏度。

磁珠酶组装用于三参数端粒酶检测