In the present study, a novel extracellular laccase isolated from Geobacillus stearothermophilus ATCC 10149 was entrapped in a bionanocomposite matrix consisting of copper alginate (Cu-alginate) supplemented with the nanoclay bentonite. After optimization, this nanobiocatalyst was able to degrade up to 90% of Remazol Brilliant Blue R (RBBR) without the addition of redox mediators and retained 70% of its initial activity for at least 1440 h, equivalent to more than 288 uses. The incorporation of nanoclay allowed alginate beads to be used in alkaline pH and strengthened its mechanical properties. Besides, this thermophilic laccase was able to decolorize other structurally different synthetic dyes such as Methyl Orange, Malachite Green and Indigo Carmine. These preliminary results suggested that the nanobiocatalyst could be a suitable option for dye decolorization and be further developed for large scale bioremediation of toxic dyes.

在本研究中，从嗜热脂肪地芽孢杆菌ATCC 10149 中分离出的一种新型细胞外漆酶被包埋在由海藻酸铜（Cu-海藻酸盐）组成的生物纳米复合基质中，并辅以纳米粘土膨润土。经过优化，这种纳米生物催化剂能够在不添加氧化还原介质的情况下降解高达 90% 的 Remazol Brilliant Blue R (RBBR)，并在至少 1440 小时内保留 70% 的初始活性，相当于超过 288 次使用。纳米粘土的加入使得海藻酸盐珠可以在碱性pH下使用并增强其机械性能。此外，这种嗜热漆酶能够对其他结构不同的合成染料如甲基橙、孔雀石绿和靛蓝胭脂红进行脱色。这些初步结果表明，纳米生物催化剂可能是染料脱色的合适选择，并可进一步开发用于有毒染料的大规模生物修复。