Porcine epidemic diarrhea virus (PEDV) of the family Coronaviridae has caused substantial economic losses in the swine husbandry industry. There’s currently no specific drug available for treatment of coronaviruses or PEDV. In the current study, we use coronavirus PEDV as a model to study antiviral agents. Briefly, a fusion inhibitor tHR2, recombinant lentivirus-delivered shRNAs targeted to conserved M and N sequences, homoharringtonine (HHT), and hydroxychloroquine (HCQ) were surveyed for their antiviral effects. Treatment with HCQ at 50 μM and HHT at 150 nM reduced virus titer in TCID50 by 30 and 3.5 fold respectively, and the combination reduced virus titer in TCID50 by 200 fold. Our report demonstrates that the combination of HHT and HCQ exhibited higher antiviral activity than either HHT or HCQ exhibited. The information may contribute to the development of antiviral strategies effective in controlling PEDV infection.

中文翻译：

---

同性harringtonine，羟氯喹或shRNA的三种治疗方法及其组合在体外对冠状病毒PEDV的治疗。

冠状病毒科的猪流行性腹泻病毒（PEDV）在养猪业中造成了巨大的经济损失。目前尚无可用于治疗冠状病毒或PEDV的特殊药物。在当前的研究中，我们使用冠状病毒PEDV作为研究抗病毒药物的模型。简而言之，调查了融合抑制剂tHR2，靶向保守M和N序列的重组慢病毒递送的shRNA，同型harringtonine（HHT）和羟氯喹（HCQ）的抗病毒作用。用50μM的HCQ和150 nM的HHT处理分别使TCID50中的病毒效价降低30倍和3.5倍，并且该组合使TCID50中的病毒效价降低200倍。我们的报告表明，HHT和HCQ的组合显示出比HHT或HCQ更高的抗病毒活性。