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Multigenerational Mitigating Effects of Ocean Acidification on In Vivo Endpoints, Antioxidant Defense, DNA Damage Response, and Epigenetic Modification in an Asexual Monogonont Rotifer.
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-06-03 , DOI: 10.1021/acs.est.0c01438 Young Hwan Lee 1 , Hye-Min Kang 1 , Min-Sub Kim 1 , Jin-Sol Lee 1 , Minghua Wang 2 , Atsushi Hagiwara 3, 4 , Chang-Bum Jeong 1, 5 , Jae-Seong Lee 1
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-06-03 , DOI: 10.1021/acs.est.0c01438 Young Hwan Lee 1 , Hye-Min Kang 1 , Min-Sub Kim 1 , Jin-Sol Lee 1 , Minghua Wang 2 , Atsushi Hagiwara 3, 4 , Chang-Bum Jeong 1, 5 , Jae-Seong Lee 1
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Ocean acidification (OA) is caused by changes in ocean carbon chemistry due to increased atmospheric pCO2 and is predicted to have deleterious effects on marine ecosystems. While the potential impacts of OA on many marine species have been studied, the multigenerational effects on asexual organisms remain unknown. We found that low seawater pH induced oxidative stress and DNA damage, decreasing growth rates, fecundity, and lifespans in the parental generation, whereas deleterious effects on in vivo endpoints in F1 and F2 offspring were less evident. The findings suggest that multigenerational adaptive effects play a role in antioxidant abilities and other defense mechanisms. OA-induced DNA damage, including double-strand breaks (DSBs), was fully repaired in F1 offspring of parents exposed to OA for 7 days, indicating that an adaptation mechanism may be the major driving force behind multigenerational adaptive effects. Analysis of epigenetic modification in response to OA involved examination of histone modification of DNA repair genes and a chromatin immunoprecipitation assay, as Bombus koreanus has no methylation pattern for CpG in its genome. We conclude that DSBs, DNA repair, and histone modification play important roles in multigenerational plasticity in response to OA in an asexual monogonont rotifer.
中文翻译：
海洋酸化对无性单性轮虫的体内端点，抗氧化防御，DNA损伤反应和表观遗传修饰的多代缓解效果。
海洋酸化（OA）是由于大气中p CO 2含量增加引起的海洋碳化学变化引起的，预计会对海洋生态系统产生有害影响。虽然已经研究了OA对许多海洋物种的潜在影响，但对无性生物的多代影响仍然未知。我们发现低海水pH值会导致氧化应激和DNA损伤，降低父母代的生长速率，繁殖力和寿命，而对体内的有害影响F1和F2后代的终点不太明显。这些发现表明，多代适应性效应在抗氧化能力和其他防御机制中起作用。OA诱导的DNA损伤，包括双链断裂（DSBs），在暴露于OA的父母的F1后代中已完全修复了7天，这表明适应机制可能是多代适应效应的主要驱动力。响应于OA的表观遗传修饰分析涉及检查DNA修复基因的组蛋白修饰和染色质免疫沉淀测定，如Bombus koreanus在其基因组中没有CpG的甲基化模式。我们得出的结论是，DSBs，DNA修复和组蛋白修饰在无性单性轮虫的OA响应多代可塑性中起着重要作用。
更新日期：2020-07-07
中文翻译：
海洋酸化对无性单性轮虫的体内端点，抗氧化防御，DNA损伤反应和表观遗传修饰的多代缓解效果。
海洋酸化（OA）是由于大气中p CO 2含量增加引起的海洋碳化学变化引起的，预计会对海洋生态系统产生有害影响。虽然已经研究了OA对许多海洋物种的潜在影响，但对无性生物的多代影响仍然未知。我们发现低海水pH值会导致氧化应激和DNA损伤，降低父母代的生长速率，繁殖力和寿命，而对体内的有害影响F1和F2后代的终点不太明显。这些发现表明，多代适应性效应在抗氧化能力和其他防御机制中起作用。OA诱导的DNA损伤，包括双链断裂（DSBs），在暴露于OA的父母的F1后代中已完全修复了7天，这表明适应机制可能是多代适应效应的主要驱动力。响应于OA的表观遗传修饰分析涉及检查DNA修复基因的组蛋白修饰和染色质免疫沉淀测定，如Bombus koreanus在其基因组中没有CpG的甲基化模式。我们得出的结论是，DSBs，DNA修复和组蛋白修饰在无性单性轮虫的OA响应多代可塑性中起着重要作用。
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