Seed germination events modulate microbial community composition, which ultimately influences seed to seedling growth performance. Here we assess the seed-borne bacteria community in disinfected and non-disinfected maize seeds and seedlings. Using a gnotobiotic system, sodium hypochlorite (1.25%, 30 min) treated-seeds showed a reduction of bacteria population size and an increase of bacteria community diversity associated with selective suppression of Burkholderia related taxon. The shift in the bacteria community composition in disinfested-seeds negatively affects germination speed, seedling growth, and reserve mobilization rates in comparison with non-disinfected maize seeds. A synthetic bacteria community formed by twelve isolates (9 Burkholderia spp.; 2 Bacillus spp. and 1 Staphylococcus sp.) obtained from natural microbiota of maize seeds herein were capable of recovering germination and seedling growth when reintroduced in disinfected seeds. Overall results showed that changes in bacterial community composition and selective reduction of Burkholderia related members dominance interfere with germination events and initial growth of the maize plantlets. By cultivation-dependent and independent approaches, we deciphered seed-maize microbiome structure, bacterial niches location, and bacterial taxon with relevant roles in seedlings growth performance. A causal relationship between seed microbial community succession and germination performance open opportunities in seed technologies to build-up microbial communities to boost plant growth and health.

中文翻译：

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洞察玉米种子萌发过程中种子传播细菌的结构和作用

种子发芽事件调节微生物群落组成，最终影响种子到幼苗的生长性能。在这里，我们评估了消毒和未消毒的玉米种子和幼苗中的种子传播细菌群落。使用gnotobiotic系统，次氯酸钠（1.25％，30分钟）处理过的种子显示出细菌种群数量的减少和细菌群落多样性的增加，与选择性抑制伯克霍尔德氏菌相关的分类单元相关。与未消毒的玉米种子相比，在受害种子中细菌群落组成的变化会对发芽速度，幼苗生长和储备动员率产生负面影响。由十二个分离株（9个伯克霍尔德氏菌； 2个芽孢杆菌属和1个葡萄球菌属）组成的合成细菌群落。当从玉米种子的天然微生物群中获得时，如果将其重新引入消毒种子中，则能够恢复萌发和幼苗生长。总体结果表明，细菌群落组成的变化和伯克霍尔德菌相关成员优势的选择性降低会干扰发芽事件和玉米幼苗的初始生长。通过依赖于栽培的方法，我们破译了种子玉米微生物组的结构，细菌生态位和细菌分类群，它们与幼苗生长性能相关。种子微生物群落演替与发芽表现之间的因果关系为种子技术提供了建立微生物群落以促进植物生长和健康的机会。