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3-Aminophenyl Boronic Acid Functionalized Quantum-Dot-Based Ratiometric Fluorescence Sensor for the Highly Sensitive Detection of Tyrosinase Activity.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-06-02 , DOI: 10.1021/acssensors.0c00122 Man Wang 1 , Jia-Ling Xie 2 , Jun Li 1 , Yao-Yao Fan 1 , Xu Deng 1 , Hui-Ling Duan 1 , Zhi-Qi Zhang 1, 2
ACS Sensors ( IF 8.2 ) Pub Date : 2020-06-02 , DOI: 10.1021/acssensors.0c00122 Man Wang 1 , Jia-Ling Xie 2 , Jun Li 1 , Yao-Yao Fan 1 , Xu Deng 1 , Hui-Ling Duan 1 , Zhi-Qi Zhang 1, 2
Affiliation
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Using the commercially available and economical 6-hydroxycoumarin (6-HC) as the substrate, a dual-emission ratiometric fluorescence sensor was developed to detect tyrosinase (TYR) activity based on 3-aminophenyl boronic acid functionalized quantum dots (APBA-QDs). TYR can catalyze 6-HC, a monohydroxy compound, to form a fluorescence-enhancing o-hydroxy compound, 6,7-dihydroxycoumarin. Owing to the special covalent binding between the o-hydroxyl and boric acid groups, APBA-QDs react with 6,7-dihydroxycoumarin to form a five-membered ring ester dual-emission fluorescence probe for TYR. With an increase in TYR activity, the fluorescence at 675 nm originating from the QDs is gradually quenched, whereas that at 465 nm owing to 6,7-dihydroxycoumarin increases. Referencing the decreasing signal of the dual-emission probe at 675 nm to measure the increasing signal at 465 nm, a ratiometric fluorescence method was established to detect the TYR activity with high sensitivity and selectivity. Under the conditions optimized via response surface methodology, a linear range of 0–0.05 U/mL was obtained for the TYR activity. The detection limit was as low as 0.003 U/mL. This sensing strategy can also be adopted for the rapid screening of the TYR inhibitors.
中文翻译：
3-氨基苯基硼酸官能化的基于量子点的比率荧光传感器，用于酪氨酸酶活性的高灵敏度检测。
使用可商购和经济的6-羟基香豆素（6-HC）作为底物，开发了一种双发射比率荧光传感器，以基于3-氨基苯基硼酸官能化的量子点（APBA-QDs）检测酪氨酸酶（TYR）活性。TYR可以催化6-HC（一种单羟基化合物）以形成增强荧光的邻羟基化合物6,7-二羟基香豆素。由于o之间特殊的共价结合-羟基和硼酸基团，APBA-QD与6,7-二羟基香豆素反应，形成用于TYR的五元环酯双发射荧光探针。随着TYR活性的增加，源自QD的675 nm处的荧光逐渐淬灭，而由于6,7-二羟基香豆素引起的465 nm处的荧光增加。参照双发射探针在675 nm处的递减信号来测量465 nm处的递增信号，建立了一种比例荧光法，以高灵敏度和高选择性检测TYR活性。在通过响应面方法优化的条件下，TYR活性的线性范围为0-0.05 U / mL。检出限低至0.003 U / mL。该传感策略也可用于快速筛选TYR抑制剂。
更新日期：2020-06-26
中文翻译：
3-氨基苯基硼酸官能化的基于量子点的比率荧光传感器，用于酪氨酸酶活性的高灵敏度检测。
使用可商购和经济的6-羟基香豆素（6-HC）作为底物，开发了一种双发射比率荧光传感器，以基于3-氨基苯基硼酸官能化的量子点（APBA-QDs）检测酪氨酸酶（TYR）活性。TYR可以催化6-HC（一种单羟基化合物）以形成增强荧光的邻羟基化合物6,7-二羟基香豆素。由于o之间特殊的共价结合-羟基和硼酸基团，APBA-QD与6,7-二羟基香豆素反应，形成用于TYR的五元环酯双发射荧光探针。随着TYR活性的增加，源自QD的675 nm处的荧光逐渐淬灭，而由于6,7-二羟基香豆素引起的465 nm处的荧光增加。参照双发射探针在675 nm处的递减信号来测量465 nm处的递增信号，建立了一种比例荧光法，以高灵敏度和高选择性检测TYR活性。在通过响应面方法优化的条件下，TYR活性的线性范围为0-0.05 U / mL。检出限低至0.003 U / mL。该传感策略也可用于快速筛选TYR抑制剂。
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