ABSTRACT The South Africa forestry industry, covering ∼1.3 million hectares, is dependent on exotic pine and Eucalyptus species. Nursery seedlings are not inoculated with ectomycorrhizal (ECM) fungi or other beneficial microbes. Fusarium circinatum is an economically important pathogen affecting seedling survival. The purpose of this investigation was to assess the effects of ectomycorrhizal fungal inoculation on Pinus patula seedling growth and resistance to the fungal pathogen F. circinatum. Explants from ECM basidiocarps, collected from Pinus stands, were plated onto MMN medium to obtain isolates that were then verified via DNA extraction and PCR amplification and sequencing of the ITS rDNA region. These isolates were identified as Boletus edulis f. reticulatus, Lactarius quieticolor, Suillus granulatus and an unknown Suillus species. P. patula growth in the presence of the pathogen F. circinatum was significantly increased and promoted by the L. quieticolor and Suillus isolates. Preventative inoculation of seedlings in the nursery using these isolates would ensure the production of stronger, healthier plants that would be more resistant to F. circinatum infection, increasing survival in the plantation.

中文翻译：

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外生菌根真菌对藤黄松幼苗上圆镰刀菌的生物防治潜力

摘要 南非林业占地约 130 万公顷，依赖于外来松树和桉树物种。苗圃幼苗未接种外生菌根 (ECM) 真菌或其他有益微生物。Fusarium circinatum 是影响幼苗存活的重要经济病原体。本研究的目的是评估外生菌根真菌接种对松树幼苗生长和对真菌病原体 F. circinatum 的抗性的影响。从松林收集的 ECM 担子果的外植体接种到 MMN 培养基上以获得分离物，然后通过 DNA 提取和 PCR 扩增以及 ITS rDNA 区域的测序进行验证。这些分离株被鉴定为牛肝菌 f。reticulatus、Lactarius quieticolor、Suillus granulatus 和一种未知的 Suillus 物种。P。在病原体 F. circinatum 存在下，L. quieticolor 和 Suillus 分离株显着增加和促进了 patula 的生长。使用这些分离株对苗圃中的幼苗进行预防性接种将确保生产更强壮、更健康的植物，这些植物将更能抵抗轮状镰刀菌感染，从而提高种植园的存活率。