Abstract This study was conducted to investigate the in vitro and in vivo antioxidant effect of Artemisia argyi powder (AAP). 240 mixed-sex one-day-old Arbor Acres broilers were randomly divided into five treatment groups, each consisting of six replicates (one replicate per cage) with eight broilers per replicate. Broilers were fed basal diets supplemented with 0, 2.5, 5, 10 and 20 g AAP per kg feed, respectively. The hepatic and intestinal samples were collected on d 21 and 42 for analysis of antioxidant indices and antioxidative enzyme gene expression. The in vitro results showed that the scavenging activity of Artemisia argyi against •OH and DPPH were 34.99±1.11% and 74.12±0.50%, respectively; the ferric reducing power was 2.58±0.03%. The in vivo results showed that dietary 20 g/kg of AAP significantly enhanced the hepatic total antioxidant capacity (T-AOC), catalase (CAT) activity, and glutathione peroxidase (GSH-Px) activity, also decreased the malondialdehyde (MDA) content; dietary10 g/kg of AAP significantly increased the gene expression of superoxide dismutase (SOD) and CAT on d 42. For the duodenum, 10 g/kg of AAP increased SOD activity (P<0.05), and reduced MDA level (P<0.05) on d 21; the gene expression of CAT and SOD were increased in the 20 g/kg of AAP treatment compared with the control group on d 42. For the jejunum, on d 21, the T-AOC level was increased by inclusion of 10 g/kg of AAP, and CAT activity was enhanced significantly at 5, 10, and 20 g/kg of AAP group; dietary AAP significantly decreased MDA level at the concentration of 2.5, 5, 10 and 20 g/kg in contrast with control group on d 42; 5 and 20 g/kg of AAP increased the gene expression of SOD on d 21, and the gene expression of GSH-Px was increased (P<0.05) in 10 g/kg of AAP group on d 42. For the ileum, compared to the control group, 2.5 and 20 g/kg of AAP increased SOD activity (P<0.05); and dietary 10 and 20 g/kg of AAP significantly reduced MDA level; dietary 10 g/kg of AAP increased the gene expression of SOD, CAT and GSH-Px in broilers on d 42. In conclusion, dietary AAP could improve the antioxidant defenses of liver and small intestine, and the best concentration of the AAP improving hepatic and small intestinal antioxidant status was 20 g/kg and 10 g/kg, respectively.

中文翻译：

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艾蒿粉体外抗氧化活性及对肉鸡肝肠抗氧化指标的影响

摘要 本研究旨在研究艾蒿粉（AAP）的体外和体内抗氧化作用。将240只混性1日龄Arbor Acres肉鸡随机分为5个处理组，每组6个重复（每笼1个重复），每个重复8只肉鸡。肉鸡饲喂基础日粮，每公斤饲料分别添加 0、2.5、5、10 和 20 克 AAP。在第 21 天和第 42 天收集肝脏和肠道样本，用于分析抗氧化指标和抗氧化酶基因表达。体外结果表明，艾蒿对•OH和DPPH的清除活性分别为34.99±1.11%和74.12±0.50%；三价铁还原能力为2.58±0.03%。体内实验结果表明，日粮20 g/kg AAP显着增强肝脏总抗氧化能力（T-AOC）、过氧化氢酶（CAT）活性和谷胱甘肽过氧化物酶（GSH-Px）活性，同时降低丙二醛（MDA）含量; 日粮10 g/kg AAP显着增加了第42天超氧化物歧化酶（SOD）和CAT的基因表达。对于十二指肠，10 g/kg AAP增加了SOD活性（P<0.05），降低了MDA水平（P<0.05） ) 第 21 天；CAT 和 SOD 的基因表达在第 42 天与对照组相比在 20 g/kg 的 AAP 处理中增加。对于空肠，在第 21 天，T-AOC 水平通过包含 10 g/kg 的AAP、CAT活性在5、10、20 g/kg AAP组显着增强；日粮 AAP 在 2.5、5、与对照组相比，第 42 天为 10 和 20 g/kg；5、20 g/kg AAP组第21天SOD基因表达增加，10 g/kg AAP组第42天GSH-Px基因表达增加（P<0.05）。与对照组相比，2.5和20 g/kg的AAP增加了SOD活性（P<0.05）；膳食中 10 和 20 g/kg 的 AAP 显着降低 MDA 水平；日粮 10 g/kg 的 AAP 增加了肉鸡第 42 天 SOD、CAT 和 GSH-Px 的基因表达。和小肠抗氧化状态分别为 20 g/kg 和 10 g/kg。10 g/kg AAP组在第42天GSH-Px基因表达增加（P<0.05）。对于回肠，与对照组相比，2.5和20 g/kg AAP组SOD活性增加（P <0.05); 膳食中 10 和 20 g/kg 的 AAP 显着降低 MDA 水平；日粮 10 g/kg 的 AAP 增加了肉鸡第 42 天 SOD、CAT 和 GSH-Px 的基因表达。和小肠抗氧化状态分别为 20 g/kg 和 10 g/kg。10 g/kg AAP组在第42天GSH-Px基因表达增加（P<0.05）。对于回肠，与对照组相比，2.5和20 g/kg AAP组SOD活性增加（P <0.05); 膳食中 10 和 20 g/kg 的 AAP 显着降低 MDA 水平；日粮 10 g/kg 的 AAP 增加了肉鸡第 42 天 SOD、CAT 和 GSH-Px 的基因表达。和小肠抗氧化状态分别为 20 g/kg 和 10 g/kg。