Tree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitised population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect nuts in processed foods is advisable. Real-Time PCR allowed a specific and accurate amplification of allergen sequences. Some food processing methods could induce structural and/or conformational changes in proteins by altering their allergenic capacity, as well as produce the fragmentation and/or degradation of genomic DNA. In this work, we analysed by means of Real-Time PCR, the influence of pressure and thermal processing through Instant Controlled Pressure Drop (DIC) on the detectability of hazelnut, pistachio and cashew allergens. The detection of targets in hazelnut, pistachio and cashew (Cor a 9, Pis v 1 and Ana o 1, respectively) is affected by the treatment to different extents depending on the tree nut. Results are compared to those previously obtained by our group in the analysis of different treatments on the amplificability of the same targets. Reduction in amplificability is similar to that reported for some autoclave conditions. Our assays might allow for the detection of up to 1000 mg/kg of hazelnut, pistachio and cashew flours after being submitted to DIC treatment in food matrices.

中文翻译：

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即时控制压降 (DIC) 处理对实时 PCR 检测坚果过敏原的影响。


坚果具有营养特性和人类健康益处。然而，它们含有过敏性蛋白质，这使得它们对敏感人群有害。食品标签上的坚果含量是强制性的，因此，建议开发合适的分析方法来检测加工食品中的坚果。实时 PCR 可以特异性、准确地扩增过敏原序列。一些食品加工方法可以通过改变蛋白质的致敏能力来诱导蛋白质的结构和/或构象变化，以及产生基因组 DNA 的片段化和/或降解。在这项工作中，我们通过实时 PCR 分析了通过即时控制压降 (DIC) 进行的压力和热处理对榛子、开心果和腰果过敏原可检测性的影响。榛子、开心果和腰果（分别为 Cor a 9、Pis v 1 和 Ana o 1）中目标的检测会受到不同程度的处理影响，具体取决于树坚果。将结果与我们小组之前在分析相同目标的不同治疗方法时获得的结果进行比较。扩增能力的降低与某些高压釜条件下报道的类似。我们的检测可在食品基质中进行 DIC 处理后检测出高达 1000 mg/kg 的榛子、开心果和腰果粉。