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Long‐term repeatability of behaviours in zebrafish (Danio rerio)
Ethology ( IF 1.3 ) Pub Date : 2020-06-03 , DOI: 10.1111/eth.13038
Hilary R. Thomson 1 , Simon D. Lamb 1 , Anne A. Besson 1 , Sheri L. Johnson 1
Affiliation  

Animal personality is defined as individual variation in behaviour that is consistent over time and/or across contexts. Animal personality is now considered a fundamental aspect in the fields of animal behaviour and behavioural ecology, yet the majority of studies assess repeatability of behaviours over only relatively short time periods (e.g. a week) using just two measures. Understanding whether behavioural traits are repeatable over longer periods is important for the assessment of individual differences in behaviour. Using zebrafish (Danio rerio), we investigated the repeatability of activity and exploratory behaviours, including distance travelled, time spent in the bottom of the arena, stationary time and overall exploration of the novel arena over a 28‐week period, using five intervals. All measures were repeatable over 28 weeks, but the repeatability estimates were much lower when comparing the initial week one and week two behaviours. There were clear sex differences in aspects of activity, with males more active than females. Importantly, our behavioural assays suggest that zebrafish require an initial “tank experience,” prior to the main phenotyping session, to ensure that behaviours being measured are repeatable—these effects are often not considered, but have implications for the many studies that measure behaviour at a single time point only.

中文翻译:

斑马鱼行为的长期可重复性(Danio rerio)

动物性格被定义为行为的个体变化,其随时间和/或环境的不同而保持一致。现在,动物性格被认为是动物行为和行为生态学领域中的一个基本方面,但是大多数研究仅使用两种方法来评估行为在较短时间内(例如一周)的可重复性。了解行为特征在更长的时期内是否可重复对于评估个人行为差异很重要。使用斑马鱼(Danio rerio),我们调查了活动和探索性行为的可重复性,包括使用距离间隔,在竞技场底部花费的时间,固定时间以及在28周内对新竞技场的整体探索(使用五个间隔)。所有测量在28周内都是可重复的,但是当比较第一周和第二周的行为时,可重复性估计值要低得多。在活动方面存在明显的性别差异,男性比女性活跃。重要的是,我们的行为分析表明,斑马鱼需要在主要的表型研究阶段之前有一个初步的“坦克经验”,以确保所测量的行为是可重复的—通常不会考虑这些影响,但会对许多测量行为的研究产生影响。仅单个时间点。
更新日期:2020-06-03
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