Monitoring is indispensable for the optimization and simulation of biotechnological processes. Hairy roots (hr, plant tissue cultures) are producers of valuable relevant secondary metabolites. The genetically stable cultures are characterized by a rapid filamentous growth, making monitoring difficult with standard methods. This article focuses on the application of laser speckle photometry (LSP) as an innovative, non‐invasive method to characterize Beta vulgaris (hr). LSP is based on the analysis of time‐resolved interference patterns. Speckle interference patterns of a biological object, known as biospeckles, are characterized by a dynamic behavior that is induced by physical and biological phenomena related to the object. Speckle contrast, a means of measuring the dynamic behavior of biospeckles, was used to assess the biospeckle activity. The biospeckle activity corresponds to processes modifying the object and correlates with the biomass growth. Furthermore, the stage of the cultures’ physiological development was assessed by speckle contrast due to the differentiation between active and low active behavior. This method is a new means of monitoring and evaluating the biomass growth of filamentous cultures in real time. As a potential tool to characterize hairy roots, LSP is non‐invasive, time‐saving, can be used online and stands out for its simple, low‐cost setup.

中文翻译：

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使用激光散斑光度测定法对毛状根培养物进行生物散斑表征


监测对于生物技术过程的优化和模拟是不可或缺的。毛状根（hr，植物组织培养物）是有价值的相关次生代谢物的生产者。遗传稳定的培养物的特点是快速丝状生长，使得用标准方法进行监测变得困难。本文重点介绍激光散斑光度测定 (LSP) 作为一种创新的非侵入性方法来表征β病毒 (hr) 的应用。 LSP 基于时间分辨干涉图样的分析。生物物体的散斑干涉图案（称为生物散斑）的特征是由与物体相关的物理和生物现象引起的动态行为。散斑对比度是一种测量生物散斑动态行为的方法，用于评估生物散斑活性。生物斑点活动对应于改变物体的过程并与生物量生长相关。此外，由于活跃和低活跃行为之间的差异，通过散斑对比度评估了培养物的生理发育阶段。该方法是实时监测和评估丝状培养物生物量生长的新手段。作为表征毛状根的潜在工具，LSP 具有非侵入性、节省时间、可在线使用，并以其简单、低成本的设置而脱颖而出。