Macrobrachium rosenbergii (M. rosenbergii) is an important freshwater cultured prawn with sexual dimorphism. The mechanism of sexual characteristic formation and sex regulation has been poorly understood. One of the greatest challenges is how to verify the physiological function of sex determination and differentiation genes by animal experiments. This paper began with detailed description of the development of the primary sexual characteristics (genital pores and male appendages) of M. rosenbergii by morphological observation and sex molecular marker technique. Secondly, the histomorphology and histology of reproductive system of M. rosenbergii were comprehensively analysed and compared. This paper firstly gave a brief overview of the earliest period, about 2.5 cm in body length, for sex identification and animal experiments on sex determination and differentiation in prawn. This study provided a convenient and noninvasive method for sex identification in the early stage of juvenile prawns.

中文翻译：

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罗氏沼虾对虾的性特征发育和性别鉴定

罗氏沼虾（Rogerbrachium rosenbergii）（M. rosenbergii）是一种重要的淡水养殖虾，具有性二态性。对性特征形成和性别调节的机制了解甚少。最大的挑战之一是如何通过动物实验来验证性别决​​定和分化基因的生理功能。本文首先通过形态学观察和性分子标记技术详细描述了罗氏沼虾的主要性特征（生殖孔和雄性附件）的发展。其次，罗氏沼虾生殖系统的组织形态学和组织学进行了全面的分析和比较。本文首先简要介绍了大约2.5厘米长的最早的时​​期，用于性别鉴定和对虾性别确定和分化的动物实验。该研究为幼虾的早期性别识别提供了一种方便且无创的方法。