Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440.,Biotechnology Journal

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Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440.
Biotechnology Journal ( IF 3.2 ) Pub Date : 2020-06-02 , DOI: 10.1002/biot.201900571
James J Williamson ₁ , Nurfariza Bahrin ₁ , Elizabeth M Hardiman ₁ , Timothy D H Bugg ₁

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Ferulic acid is a renewable chemical found in lignocellulose from grasses such as wheat straw and sugarcane. Pseudomonas putida is able to liberate and metabolize ferulic acid from plant biomass. Deletion of the hydroxycinnamoyl‐CoA hydratase‐lyase gene (ech ) produced a strain of P. putida unable to utilize ferulic and p‐coumaric acid, which is able to accumulate ferulic acid and p‐coumaric acid from wheat straw or sugar cane bagasse. Further engineering of this strain saw the replacement of ech with the phenolic acid decarboxylase padC , which converts p‐coumaric and ferulic acid into 4‐vinylphenol and the flavor agent 4‐vinylguaiacol, respectively. The engineered strain containing padC is able to generate 4‐vinylguaiacol and 4‐vinylphenol from media containing lignocellulose or Green Value Protobind lignin as feedstock, and does not require the addition of an exogenous inducer molecule. Biopolymerization of 4‐vinylguaiacol and 4‐vinylcatechol styrene products is also carried out, using Trametes versicolor laccase, to generate “biopolystyrene” materials on small scale.

中文翻译：

使用工程假单胞菌假单胞菌KT2440从木质素和木质纤维素生产取代的苯乙烯生物产品。

阿魏酸是木质纤维素中的一种可再生化学物质，来自麦草和甘蔗等草。恶臭假单胞菌能够从植物生物质中释放和代谢阿魏酸。羟肉桂酰辅酶A水合酶裂解酶基因（ech）的缺失导致恶臭假单胞菌无法利用阿魏酸和对香豆酸，该菌株能够从小麦秸秆或甘蔗渣中积累阿魏酸和对香豆酸。对该菌株的进一步工程研究发现，ech被酚酸脱羧酶padC取代，后者将对香豆酸和阿魏酸分别转化为4-乙烯基苯酚和风味剂4-乙烯基愈创木酚。含有padC的工程菌株能够从含有木质纤维素或Green Value Protobind木质素作为原料的培养基中产生4-乙烯基愈创木酚和4-乙烯基苯酚，并且不需要添加外源诱导剂分子。还使用Trametes versicolor漆酶对4-乙烯基愈创木酚和4-乙烯基邻苯二酚苯乙烯产品进行生物聚合，以小规模生产“生物聚苯乙烯”材料。

更新日期：2020-07-08

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