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The majority of the matrix protein TapA is dispensable for Bacillus subtilis colony biofilm architecture.
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-06-03 , DOI: 10.1111/mmi.14559
Chris Earl 1 , Sofia Arnaouteli 1 , Natalie C Bamford 1 , Michael Porter 1 , Tetyana Sukhodub 1 , Cait E MacPhee 2 , Nicola R Stanley-Wall 1
Affiliation  

Biofilm formation is a co‐operative behaviour, where microbial cells become embedded in an extracellular matrix. This biomolecular matrix helps manifest the beneficial or detrimental outcome mediated by the collective of cells. Bacillus subtilis is an important bacterium for understanding the principles of biofilm formation. The protein components of the B. subtilis matrix include the secreted proteins BslA, which forms a hydrophobic coat over the biofilm, and TasA, which forms protease‐resistant fibres needed for structuring. TapA is a secreted protein also needed for biofilm formation and helps in vivo TasA‐fibre formation but is dispensable for in vitro TasA‐fibre assembly. We show that TapA is subjected to proteolytic cleavage in the colony biofilm and that only the first 57 amino acids of the 253‐amino acid protein are required for colony biofilm architecture. Through the construction of a strain which lacks all eight extracellular proteases, we show that proteolytic cleavage by these enzymes is not a prerequisite for TapA function. It remains unknown why TapA is synthesised at 253 amino acids when the first 57 are sufficient for colony biofilm structuring; the findings do not exclude the core conserved region of TapA having a second role beyond structuring the B. subtilis colony biofilm.

中文翻译:

大多数基质蛋白 TapA 对于枯草芽孢杆菌菌落生物膜结构是可有可无的。

生物膜形成是一种合作行为,其中微生物细胞嵌入细胞外基质中。这种生物分子基质有助于表现出由细胞集合介导的有益或有害结果。枯草芽孢杆菌是了解生物膜形成原理的重要细菌。枯草芽孢杆菌的蛋白质成分基质包括分泌蛋白 BslA,它在生物膜上形成疏水涂层,以及 TasA,它形成构建所需的蛋白酶抗性纤维。TapA 是一种分泌蛋白,也是生物膜形成所必需的,有助于体内 TasA 纤维的形成,但对于体外 TasA 纤维组装来说是不必要的。我们表明,TapA 在菌落生物膜中受到蛋白水解切割,并且菌落生物膜结构只需要 253 个氨基酸蛋白质的前 57 个氨基酸。通过构建缺乏所有八种细胞外蛋白酶的菌株,我们表明这些酶的蛋白水解切割不是 TapA 功能的先决条件。当前 57 个氨基酸足以形成菌落生物膜时,为什么 TapA 在 253 个氨基酸处被合成仍然未知;B. 枯草菌落生物膜。
更新日期:2020-06-03
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