The ethanolic extracts of many plants have been used in alternative medicine. The present study aimed at evaluating the antioxidant, cytotoxicity, and anticancer potential of cactus and lupin ethanolic extracts compared to utoral drug (UT) on the colon Caco‐2 cancer cell line. Bioactive components, cytotoxicity of Caco‐2 cell cycle, and gene regulation of apoptosis genes were studied by HPLC, flow cytometer, and RT‐PCR, respectively. Lupin extract (LE) contained high bioactive components and antioxidant potential. The predominant phenol, flavonoid, and sterol in LE were rosmarinic acid (2,004.8 μg/ml), quercetin (9,912 µg/g), and ergosterol (2.77 µg/g). LE and its mixture with utoral showed high cytotoxicity and effective potential in regulation of gene expression of proapoptotic and antiapoptotic genes in Caco‐2 cells. In conclusion, LE and cactus extract (CE) could be considered as natural preparations with high anticancer properties against Caco‐2 cells. LE had the highest anticancer potential among the tested preparations.

中文翻译：

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与常规药物相比，仙人掌和羽扇豆提取物可作为潜在的抗癌药。

许多植物的乙醇提取物已用于替代医学。本研究旨在评估与结肠癌Caco-2癌细胞系上的常规药物（UT）相比，仙人掌和羽扇豆乙醇提取物的抗氧化剂，细胞毒性和抗癌潜力。通过HPLC，流式细胞仪和RT-PCR分别研究了Caco-2细胞周期的生物活性成分，细胞毒性和凋亡基因的基因调控。羽扇豆提取物（LE）含有高生物活性成分和抗氧化潜力。LE中主要的苯酚，类黄酮和固醇为迷迭香酸（2,004.8μg/ ml），槲皮素（9,912μg/ g）和麦角固醇（2.77μg/ g）。LE及其与toalal的混合物在调节Caco-2细胞中促凋亡和抗凋亡基因的基因表达方面显示出高细胞毒性和有效潜力。结论，LE和仙人掌提取物（CE）可以被视为对Caco-2细胞具有高度抗癌特性的天然制剂。LE在测试的制剂中具有最高的抗癌潜力。