Bone tissue engineering is a promising approach for tackling clinical challenges. Osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds are employed in bone tissue engineering. However, scaffold materials remain limited due to their source, low biocompatibility, and so on. In this study, a composite hydrogel scaffold composed of hydroxyapatite (HA) and sodium alginate (SA) was manufactured using three‐dimensional printing. Naringin (NG) and calcitonin‐gene‐related peptide (CGRP) were used as osteogenic factors in the fabrication of drug‐loaded scaffolds. Investigation using animal experiments, as well as scanning electron microscopy, cell counting kit‐8 testing, alkaline phosphatase staining, and alizarin red‐D staining of bone marrow mesenchymal stem cell culture showed that the three scaffolds displayed similar physicochemical properties and that the HA/SA/NG and HA/SA/CGRP scaffolds displayed better osteogenesis than that of the HA/SA scaffold. Thus, the HA/SA scaffold could be a biocompatible material with potential applications in bone regeneration. Meanwhile, NG and CGRP doping could result in better and more positive proliferation and differentiation.

中文翻译：

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基于羟基磷灰石-海藻酸钠的载药三维支架用于骨再生。

骨组织工程是应对临床挑战的一种很有前景的方法。骨祖细胞、成骨因子和骨诱导/骨传导支架用于骨组织工程。然而，支架材料由于其来源、低生物相容性等原因仍然有限。在这项研究中，使用三维打印制造了由羟基磷灰石 (HA) 和海藻酸钠 (SA) 组成的复合水凝胶支架。柚皮素（NG）和降钙素基因相关肽（CGRP）被用作制备载药支架的成骨因子。使用动物实验进行调查，以及扫描电子显微镜、细胞计数试剂盒-8 测试、碱性磷酸酶染色、骨髓间充质干细胞培养物的茜素红-D染色显示三种支架显示出相似的理化特性，并且HA/SA/NG和HA/SA/CGRP支架显示出比HA/SA支架更好的成骨作用。因此，HA/SA 支架可能是一种在骨再生中具有潜在应用的生物相容性材料。同时，NG 和 CGRP 掺杂可以导致更好和更积极的增殖和分化。