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Analysis of Modified Nucleotide Aptamer Library Generated by Thermophilic DNA Polymerases.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-06-03 , DOI: 10.1002/cbic.202000236
Krisztina Percze 1 , Tamás Mészáros 1
Affiliation  

To select aptamers with modified nucleotides, the appropriate DNA polymerase must be designated. We studied eight polymerases and demonstrated that incorporating a tryptophan‐like nucleotide into aptamer libraries by emulsion PCR and KOD XL had an insignificant effect on the PCR bias and did not distort the sequence space, thus making this approach the method of choice.
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中文翻译:

嗜热DNA聚合酶产生的修饰核苷酸适体文库的分析。

要选择核苷酸修饰的适体,必须指定适当的DNA聚合酶。我们研究了8种聚合酶,并证明通过乳液PCR和KOD XL将色氨酸样核苷酸掺入适体文库中对PCR的影响不显着,并且不会扭曲序列空间,因此使该方法成为了选择的方法。
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更新日期:2020-06-03
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