Abstract A method for micropropagation of Coccoloba uvifera (L.) L. has been developed through bud proliferation from surface-sterilized nodal explants collected from a 9 yr old mature tree. Ninety six percent of the explants exhibited bud development on agar-gelled Murashige and Skoog's (MS) medium supplemented with 3.0 mg L−1 6-benzylaminopurine (BAP) if incubated at 26 ± 2 °C, under 40–50 µmol m−2 s−1 spectral photon flux density (SPFD) light intensity for 16/8 h light/dark photoperiod and 55–60% relative humidity. The cloned buds multiplied (12 ± 1.30 shoots per inoculum), and grew on MS medium + 1.0 mg L−1 BAP + 0.5 mg L−1 of α-Naphthalene acetic acid (NAA) and additives, 50 mg L−1 ascorbic acid, 25 mg L−1 each of adenine sulphate, citric acid and arginine. Higher concentrations of auxins in the culture medium induced callus formation. The additives improved quality of shoots as these prevented deformation of shoots. The shoots rooted (7.2 roots per shoot) on half-strength MS medium with 2.5 mg L−1 NAA. Ex vitro rooting was achieved by treating the basal parts of the in vitro shoots with 400 mg L−1 NAA (14.6 roots per shoot) and subsequent planting in pots filled with 1:1:1 mixture of soilrite® (soil conditioning mixture), cocopeat and garden soil. The cloned plantlets were acclimatized in greenhouse; 93.8% of these were survived. This tissue culture method can be applied for large scale production and sustainable conservation of C. uvifera, a vulnerable species for agroforestry of the coastal regions for windbreak and soil reclamations.

中文翻译：

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海葡萄（Coccoloba uvifera (L.) L.）的微繁殖

摘要 一种用于 Coccoloba uvifera (L.) L. 微繁殖的方法已经通过从 9 岁成熟树收集的表面消毒的节点外植体的芽增殖而开发。如果在 26 ± 2 °C、40–50 µmol m-2 下培养，96% 的外植体在添加了 3.0 mg L-1 6-苄氨基嘌呤 (BAP) 的琼脂凝胶 Murashige 和 Skoog's (MS) 培养基上出现芽发育s-1 光谱光子通量密度 (SPFD) 光强度，适用于 16/8 小时光/暗光周期和 55-60% 的相对湿度。克隆芽繁殖（每个接种物 12 ± 1.30 个芽），并在 MS 培养基 + 1.0 mg L-1 BAP + 0.5 mg L-1 α-萘乙酸 (NAA) 和添加剂、50 mg L-1 抗坏血酸上生长, 硫酸腺嘌呤、柠檬酸和精氨酸各 25 mg L-1。培养基中较高浓度的生长素诱导愈伤组织形成。添加剂提高了芽的质量，因为它们防止了芽的变形。枝条在含有 2.5 mg L-1 NAA 的半强度 MS 培养基上生根（每个枝条 7.2 个根）。体外生根是通过用 400 mg L-1 NAA（每个芽 14.6 个根）处理体外芽的基部并随后种植在装有 1:1:1 土壤调节混合物（土壤调理混合物）的盆中来实现的，椰子油和花园土壤。克隆苗在温室中驯化；其中93.8%存活。这种组织培养方法可用于 C. uvifera 的大规模生产和可持续保护，C. uvifera 是沿海地区防风林和土壤开垦农林业的脆弱物种。每个芽 2 个根）在含有 2.5 mg L-1 NAA 的半强度 MS 培养基上。体外生根是通过用 400 mg L-1 NAA（每个芽 14.6 个根）处理体外芽的基部并随后种植在装有 1:1:1 土壤调节混合物（土壤调理混合物）的盆中来实现的，椰子油和花园土壤。克隆苗在温室中驯化；其中93.8%存活。这种组织培养方法可用于 C. uvifera 的大规模生产和可持续保护，C. uvifera 是沿海地区防风林和土壤开垦农林业的脆弱物种。每个芽 2 个根）在含有 2.5 mg L-1 NAA 的半强度 MS 培养基上。体外生根是通过用 400 mg L-1 NAA（每个芽 14.6 个根）处理体外芽的基部并随后种植在装有 1:1:1 土壤调节混合物（土壤调理混合物）的盆中来实现的，椰子油和花园土壤。克隆苗在温室中驯化；其中93.8%存活。这种组织培养方法可用于 C. uvifera 的大规模生产和可持续保护，C. uvifera 是沿海地区防风林和土壤开垦农林业的脆弱物种。椰子油和花园土壤。克隆苗在温室中驯化；其中93.8%存活。这种组织培养方法可用于 C. uvifera 的大规模生产和可持续保护，C. uvifera 是沿海地区防风林和土壤开垦农林业的脆弱物种。椰子油和花园土壤。克隆苗在温室中驯化；其中93.8%存活。这种组织培养方法可用于 C. uvifera 的大规模生产和可持续保护，C. uvifera 是沿海地区防风林和土壤开垦农林业的脆弱物种。