Cyclic lipopeptides (CLPs) from Bacillus strains have demonstrated a wide range of bioactivities making them interesting candidates for different applications in the pharmaceutical, food and biotechnological industries. Genome sequencing, together with phylogenetic analysis of the Bacillus sp. P34, isolated from a freshwater fish gut, showed that the bacterial strain belongs to the Bacillus velezensis group. In silico investigation of metabolic gene clusters of nonribosomal peptide synthetases (NRPS) revealed the genetic elements associated with the synthesis of surfactin, fengycin and iturin family component bacillomycin. Further, an assay was conducted to investigate the production of CLPs in the presence of heat inactivated bacterial cultures or fungal spores. Maximum fengycin concentration was observed at 24 h (2300−2700 mg/mL), while maximum iturin amounts were detected at 48 h (250 mg/mL) in the presence of heat-inactivated spores of Aspergillus niger. Heat-inactivated cells of Listeria monocytogenes caused a reduction of both fengycin and iturin amounts. The production of fengycins A and B and the iturin family component bacillomycin L was confirmed by mass spectrometry analyses. This study reinforces the potential of B. velezensis P34 as a valuable strain for biotechnological production of CLPs recognized as important antimicrobial substances.

来自芽孢杆菌属菌株的环状脂肽（CLP）已显示出广泛的生物活性，使其成为制药，食品和生物技术行业不同应用的有趣候选物。基因组测序，以及芽孢杆菌的系统发育分析。从淡水鱼肠中分离出的P34表明该细菌菌株属于Velacensis芽孢杆菌。电脑对非核糖体肽合成酶（NRPS）的代谢基因簇的研究揭示了与表面活性素，丰霉素和伊图林家族成分杆菌素合成相关的遗传因素。此外，进行了测定以研究在热灭活细菌培养物或真菌孢子存在下CLP的产生。在黑曲霉热灭活的孢子存在下，在24 h（2300−2700 mg / mL）观察到最大的风霉素浓度，而在48 h（250 mg / mL）观察到最大的尿素含量。单核细胞增生李斯特菌的热灭活细胞导致风霉素和尿素的含量均降低。通过质谱分析证实了丰霉素A和B以及伊图林家族成分杆菌霉素L的产生。这项研究增强了velezensis P34作为作为公认的重要抗微生物物质的CLP生物技术生产的有价值菌株的潜力。