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Mapping quantitative trait loci associated with starch paste viscosity attributes by using double haploid populations of rice (Oryza sativa L.)
Journal of Integrative Agriculture ( IF 4.8 ) Pub Date : 2020-06-02 , DOI: 10.1016/s2095-3119(19)62726-7
Tahmina SHAR , Zhong-hua SHENG , Umed ALI , Sajid FIAZ , Xiang-jin WEI , Li-hong XIE , Gui-ai JIAO , Fahad ALI , Gao-neng SHAO , Shi-kai HU , Pei-song HU , Shao-qing TANG

The paste viscosity attributes of starch, measured by rapid visco analyzer (RVA), are important factors for the evaluation of the cooking and eating qualities of rice in breeding programs. To determine the genetic roots of the paste viscosity attributes of rice grains, quantitative trait loci (QTLs) associated with the paste viscosity attributes were mapped, using a double haploid (DH) population derived from Zhongjiazao 17 (YK17), a super rice variety, crossed with D50, a tropic japonica variety. Fifty-four QTLs, for seven parameters of the RVA profiles, were identified in three planting seasons. The 54 QTLs were located on all of the 12 chromosomes, with a single QTL explaining 5.99 to 47.11% of phenotypic variation. From the QTLs identified, four were repeatedly detected under three environmental conditions and the other four QTLs were repeated under two environments. Most of the QTLs detected for peak viscosity (PKV), trough viscosity (TV), cool paste viscosity (CPV), breakdown viscosity (BDV), setback viscosity (SBV), and peak time (PeT) were located in the interval of RM6775–RM3805 under all three environmental conditions, with the exception of pasting temperature (PaT). For digenic interactions, eight QTLs with six traits were identified for additive×environment interactions in all three planting environments. The epistatic interactions were estimated only for PKV, SBV and PaT. The present study will facilitate further understanding of the genetic architecture of eating and cooking quality (ECQ) in the rice quality improvement program.



中文翻译:

映射数量性状基因座与淀粉糊粘度相关联的通过使用水稻的双单倍体群体属性(L.)

通过快速粘度分析仪(RVA)测量的淀粉糊的粘度属性,是评估育种程序中大米的烹饪和食用品质的重要因素。为了确定稻米糊状黏度属性的遗传根源,使用超级稻品种仲家早17(YK17)的双单倍体(DH)群体绘制了与糊状黏度属性相关的定量性状位点(QTL),与热带粳稻D50杂交品种。在三个种植季节中,确定了RVA分布图的七个参数的五十四个QTL。54个QTL位于12条染色体的全部上,其中一个QTL解释了5.99%至47.11%的表型变异。从确定的QTL中,在三个环境条件下重复检测了四个,在四个环境中重复了其他四个QTL。检测到的大多数QTL的峰值粘度(PKV),谷底粘度(TV),冷浆粘度(CPV),击穿粘度(BDV),后退粘度(SBV)和峰值时间(PeT)位于RM6775的范围内–在所有三种环境条件下的RM3805,粘贴温度(PaT)除外。对于双基因互作,在所有三种种植环境中,确定了具有六个性状的八个QTL用于加性x环境互作。仅对PKV,SBV和PaT估计上位相互作用。本研究将促进对稻米品质改良计划中饮食和烹饪品质(ECQ)基因结构的进一步了解。

更新日期:2020-06-02
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