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Genome-wide identification and transcriptome profiling reveal great expansion of SWEET gene family and their wide-spread responses to abiotic stress in wheat (Triticum aestivum L.)
Journal of Integrative Agriculture ( IF 4.8 ) Pub Date : 2020-06-02 , DOI: 10.1016/s2095-3119(19)62761-9
Jin-xia QIN , Yu-jie JIANG , Yun-ze LU , Peng ZHAO , Bing-jin WU , Hong-xia LI , Yu WANG , Sheng-bao XU , Qi-xin SUN , Zhen-shan LIU

The Sugars Will Eventually be Exported Transporter (SWEET) gene family, identified as sugar transporters, has been demonstrated to play key roles in phloem loading, grain filling, pollen nutrition, and plant-pathogen interactions. To date, the study of SWEET genes in response to abiotic stress is very limited. In this study, we performed a genome-wide identification of the SWEET gene family in wheat and examined their expression profiles under mutiple abiotic stresses. We identified a total of 105 wheat SWEET genes, and phylogenic analysis revealed that they fall into five clades, with clade V specific to wheat and its closely related species. Of the 105 wheat SWEET genes, 59% exhibited significant expression changes after stress treatments, including drought, heat, heat combined with drought, and salt stresses, and more up-regulated genes were found in response to drought and salt stresses. Further hierarchical clustering analysis revealed that SWEET genes exhibited differential expression patterns in response to different stress treatments or in different wheat cultivars. Moreover, different phylogenetic clades also showed distinct response to abiotic stress treatments. Finally, we found that homoeologous SWEET genes from different wheat subgenomes exhibited differential expression patterns in response to different abiotic stress treatments. The genome-wide analysis revealed the great expansion of SWEET gene family in wheat and their wide participation in abiotic stress response. The expression partitioning of SWEET homoeologs under abiotic stress conditions may confer greater flexibility for hexaploid wheat to adapt to ever changing environments.



中文翻译:

全基因组范围内的鉴定和转录组分析揭示了SWEET基因家族的巨大扩展以及它们对小麦中非生物胁迫的广泛响应(Triticum aestivum L.)

小号ugars W¯¯生病é ventually是Ë xported ŧ兰斯波特(SWEET)基因家族被确定为糖转运体,已被证明在韧皮部负载,籽粒充实,花粉营养和植物-病原体相互作用中起关键作用。迄今为止,响应非生物胁迫的SWEET基因的研究非常有限。在这项研究中,我们进行了小麦中SWEET基因家族的全基因组鉴定,并研究了其在多种非生物胁迫下的表达谱。我们共鉴定了105个小麦SWEET基因,系统发育分析表明它们属于五个进化枝,其中进化枝V特定于小麦及其紧密相关的物种。在105个小麦SWEET基因中,有59%在胁迫处理后表现出显着的表达变化,包括干旱,高温,高温与干旱和盐胁迫,并且发现了更多上调的基因来应对干旱和盐胁迫。进一步的层次聚类分析表明,SWEET基因在不同的胁迫处理或不同的小麦品种中表现出不同的表达模式。此外,不同的系统进化分支也显示出对非生物胁迫处理的独特反应。最后,我们发现来自不同小麦亚基因组的同源SWEET基因对不同的非生物胁迫处理表现出不同的表达模式。全基因组分析揭示了小麦中SWEET基因家族的巨大扩展以及它们广泛参与非生物胁迫反应。在非生物胁迫条件下,SWEET同源物的表达分区可能赋予六倍体小麦更大的灵活性,以适应不断变化的环境。进一步的层次聚类分析表明,SWEET基因在不同的胁迫处理或不同的小麦品种中表现出不同的表达模式。此外,不同的系统进化分支也显示出对非生物胁迫处理的独特反应。最后,我们发现来自不同小麦亚基因组的同源SWEET基因对不同的非生物胁迫处理表现出不同的表达模式。全基因组分析揭示了小麦中SWEET基因家族的巨大扩展以及它们广泛参与非生物胁迫反应。在非生物胁迫条件下,SWEET同源物的表达分区可能赋予六倍体小麦更大的灵活性,以适应不断变化的环境。进一步的层次聚类分析表明,SWEET基因在不同的胁迫处理或不同的小麦品种中表现出不同的表达模式。此外,不同的系统进化分支也显示出对非生物胁迫处理的独特反应。最后,我们发现来自不同小麦亚基因组的同源SWEET基因对不同的非生物胁迫处理表现出不同的表达模式。全基因组分析揭示了小麦中SWEET基因家族的巨大扩展以及它们广泛参与非生物胁迫反应。在非生物胁迫条件下,SWEET同源物的表达分区可能赋予六倍体小麦更大的灵活性,以适应不断变化的环境。

更新日期:2020-06-02
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