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Effect of three insect-resistant maizes expressing Cry1Ie, Cry1Ab/Cry2Aj and Cry1Ab on the growth and development of armyworm Mythimna separata (Walker)
Journal of Integrative Agriculture ( IF 4.6 ) Pub Date : 2020-06-02 , DOI: 10.1016/s2095-3119(20)63162-8
Hong-hua SU , Tao JIANG , Yu SUN , Hui-jie GU , Jiao-jiao WU , Yi-zhong YANG

Three transgenic maize events (IE09S034, Shuangkang 12–5 and C0030.3.5) produced Cry1le, Cry1Ab/Cry2Aj and G10-EPSPS, Cry1Ab and EPSPS, respectively, all of which target the Asian corn borer. The oriental armyworm Mythimna separata (Walker) is the secondary target. In this study, the effects of the three Bt maizes on the development and survival of armyworm were studied. The results showed that IE09S034 had insecticidal activity against 1st instar larvae, and the survival rate of armyworm fed with Bt maize for 10 days was 46.2%, significantly lower than that of the control. The larvae at 3rd–6th instar were more tolerant of the Bt toxin than the early instar larvae. However, Shuangkang 12-5 had good insecticidal activity against 1st–5th instar larvae. The mortality was nearly 100% when the larvae were fed with Shuangkang 12-5 before 3rd instar, and the toxin had quick-acting efficacy. This event significantly inhibited the development of armyworm; that is, the larval duration of the 3rd and 4th instar larvae fed with Shuangkang 12-5 was prolonged by 4.5 and 3.0 days, respectively. The pupal weight and egg number were also significantly lower than those of the control. For C0030.3.5, it could control 1st–5th instar larvae effectively. The mortality rates were all over 50% if 1st–3rd larvae were fed with this event. The pupal weight of 4th–6th instar larvae fed with Bt maize were only 53.9, 56.8 and 54.6%, respectively, compared to that of the control. The number of eggs laid was significantly less than the control. The results indicate that all three transgenic maize events exhibit the potential to provide effective control of early instar larvae of armyworm, which can be commercialized in future to control lepidoptera pests such as Asian corn borer and armyworm.



中文翻译:

三种表达Cry1Ie,Cry1Ab / Cry2Aj和Cry1Ab的抗虫玉米对粘虫Mythimna separata(Walker)生长发育的影响

三个转基因玉米事件(IE09S034,双康12-5和C0030.3.5)分别产生了Cry1le,Cry1Ab / Cry2Aj和G10-EPSPS,Cry1Ab和EPSPS,所有这些都针对亚洲玉米bore。东方粘虫Mythimna separata(行者)是次要目标。在这项研究中,研究了三种Bt玉米对粘虫生长和存活的影响。结果表明,IE09S034对一龄幼虫具有杀虫活性,饲喂Bt玉米10天的夜蛾成活率为46.2%,明显低于对照。与初龄幼虫相比,第三龄至第六龄幼虫对Bt毒素的耐受性更高。然而,双抗12-5对1至5龄幼虫具有良好的杀虫活性。在三龄前给双康12-5喂食幼虫,死亡率接近100%,且该毒素具有速效作用。这一事件大大抑制了粘虫的发展。也就是说,双康12-5喂养的三龄和四龄幼虫的幼虫期分别延长了4.5天和3.0天,分别。weight的重量和卵数也显着低于对照组。对于C0030.3.5,它可以有效控制第1至第5龄幼虫。如果给第一至第三幼虫喂食,则死亡率都超过50%。与对照相比,Bt玉米饲喂的第四至第六龄幼虫的weight重分别仅为53.9、56.8和54.6%。产卵的数量明显少于对照。结果表明,这三个转基因玉米事件均显示出有可能提供有效的防治粘虫幼龄幼虫的潜能,今后可将其商业化以防治鳞翅目害虫,例如亚洲玉米bore和粘虫。它可以有效地控制第1至第5龄幼虫。如果给第一至第三幼虫喂食,则死亡率都超过50%。与对照相比,Bt玉米饲喂的第四至第六龄幼虫的up体重分别仅为53.9、56.8和54.6%。产卵的数量明显少于对照。结果表明,这三个转基因玉米事件均显示出有可能提供有效的防治粘虫幼龄幼虫的潜力,将来可将其商业化以防治鳞翅目害虫,例如亚洲玉米bore和粘虫。它可以有效地控制第1至第5龄幼虫。如果给第一至第三幼虫喂食,则死亡率都超过50%。与对照相比,Bt玉米饲喂的第四至第六龄幼虫的up体重分别仅为53.9、56.8和54.6%。产卵数明显少于对照。结果表明,这三个转基因玉米事件均显示出有可能提供有效的防治粘虫幼龄幼虫的潜力,将来可将其商业化以防治鳞翅目害虫,例如亚洲玉米bore和粘虫。产卵数明显少于对照。结果表明,这三个转基因玉米事件均显示出有可能提供有效的防治粘虫幼龄幼虫的潜力,将来可将其商业化以防治鳞翅目害虫,例如亚洲玉米bore和粘虫。产卵的数量明显少于对照。结果表明,这三个转基因玉米事件均显示出有可能提供有效的防治粘虫幼龄幼虫的潜能,今后可将其商业化以防治鳞翅目害虫,例如亚洲玉米bore和粘虫。

更新日期:2020-06-02
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