Abstract Ammonia oxidizing bacteria (AOB) and archaea (AOA) have different affinities to different fertilizer N sources; however, there are few studies on the relative contribution of AOB and AOA to N2O emissions under different N fertilizers. In this study, acetylene (0.01%v/v C2H2) and 1-octyne (0.01%v/v) were used to assess the relative contribution of AOB and AOA to N2O emission from paddy soils with different soil pH and N fertilizers application by incubation in microcosms. The results indicated that cumulative N2O emissions from the alkaline paddy soil were significantly higher than those from the acidic paddy soil, and urea-N application promoted more N2O emissions than ammonium-N application. The total relative contribution of AOA and AOB exceeded 60%, so autotrophic nitrification was the main N2O emission process in both soils at 80% water holding capacity (WHC); after N application, their total contribution increased. The relative contribution of 1-octyne-sensitive AOB (>45%) to N2O emissions was significantly higher than that of AOA (

中文翻译：

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氨氧化细菌和古细菌对两种不同施氮源水稻土N2O排放的相对贡献：一个缩影研究

摘要 氨氧化细菌（AOB）和古细菌（AOA）对不同肥料氮源的亲和力不同；然而，关于不同氮肥下 AOB 和 AOA 对 N2O 排放的相对贡献的研究很少。在本研究中，使用乙炔 (0.01%v/v C2H2) 和 1-辛炔 (0.01%v/v) 评估 AOB 和 AOA 对不同土壤 pH 值和施氮肥的水稻土 N2O 排放的相对贡献。在微观世界中孵化。结果表明，碱性稻田的 N2O 累积排放量显着高于酸性稻田土壤的 N2O 排放量，且施用尿素氮比施用铵态氮促进了更多的 N2O 排放。AOA和AOB的总相对贡献超过60%，因此，自养硝化作用是两种土壤在 80% 持水量 (WHC) 中的主要 N2O 排放过程；N次申请后，他们的总贡献增加了。1-辛炔敏感 AOB（>45%）对 N2O 排放的相对贡献显着高于 AOA（