Anti-biofilm activity of silver nanoparticle-containing glass ionomer cements.,Dental Materials

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Anti-biofilm activity of silver nanoparticle-containing glass ionomer cements.
Dental Materials ( IF 4.6 ) Pub Date : 2020-06-03 , DOI: 10.1016/j.dental.2020.05.001
G C Porter ₁ , G R Tompkins ₁ , D R Schwass ₁ , K C Li ₁ , J N Waddell ₁ , C J Meledandri ₂

Affiliation

Objective

To develop a silver nanoparticle (AgNP) formulation for incorporation into glass ionomer cements (GICs) which minimises biofilm growth on restoration surfaces.

Methods

GICs, Fuji IX, Ketac Molar, and Riva Selfcure were modified with 6, 10 and 24 μg per GIC capsule of α-lipoic acid-capped AgNPs. Monoculture biofilms of Streptococcus mutans were cultured (72 h) on GIC specimens (n = 3) and biofilm accumulation was quantified using a viability stain with confocal laser scanning microscopy. Compression strength and flexural strength (CS & FS) were measured according to ISO 9917-1:2007 (n = 8, n = 25). GIC colour was measured at 0, 1, and 14 days following AgNP incorporation using a digital spectrophotometer. Silver release from AgNP-modified GIC specimens was monitored at 1, 3, 7 and 14 days using inductively coupled plasma-mass spectrometry.

Results

AgNP-modified Fuji IX demonstrated the greatest reduction in biofilm accumulation, with 10 μg Ag/capsule inhibiting biofilm formation by 99%. Ketac Molar and Riva Selfcure required 24 μg Ag/capsule to achieve 78% biofilm reduction. AgNP-modified GICs demonstrated significantly higher CS and FS than sintered silver-containing GICs, and possessed equivalent or higher strength values when compared to unmodified GICs. The colour shades of AgNP-modified GICs were more comparable to VITA shades of non-modified GICs than were sintered silver-containing GICs. The silver (≥99.6%) remained within the GIC for at least two weeks following incorporation.

Significance

AgNP-modified GICs exhibited significant antibiofilm activity and retained mechanical properties equivalent or superior to non-modified GICs. AgNP-modified GICs could reduce bacterial colonisation on and around restorations thereby reducing restoration failure caused by secondary caries.

中文翻译：

含银纳米粒子的玻璃离聚物水泥的抗生物膜活性。

目的

开发一种银纳米颗粒（AgNP）配方，以掺入玻璃离聚物水泥（GIC）中，从而最大程度减少修复表面的生物膜生长。

方法

GIC，Fuji IX，Ketac Molar和Riva Selfcure被修饰为每个GIC胶囊中的α-硫辛酸封端的AgNP分别为6、10和24μg。在GIC标本（n = 3）上培养（72 h）变形链球菌的单培养生物膜，并通过共聚焦激光扫描显微镜用活力染色剂对生物膜的积累进行定量。压缩强度和抗弯强度（CS和FS）根据ISO 9917-1：2007（n = 8，n = 25）进行测量。使用数字分光光度计在加入AgNP后的第0、1和14天测量GIC颜色。使用电感耦合等离子体质谱法在第1、3、7和14天监测AgNP修饰的GIC样品中的银释放。

结果

AgNP改性的Fuji IX表现出最大的生物膜积聚减少，其中10μgAg /胶囊可抑制生物膜形成99％。Ketac Molar和Riva Selfcure需要24微克Ag /胶囊才能实现78％的生物膜减少。与未经修饰的GIC相比，AgNP修饰的GIC的CS和FS明显高于烧结的含银GIC，并且具有同等或更高的强度值。与烧结的含银GIC相比，AgNP修饰的GIC的色泽与未修饰的GIC的VITA色泽更具可比性。掺入后，银（≥99.6％）保留在GIC中至少两周。