Abstract Postharvest decay and senescence results in significant losses in harvested mushrooms. Integrated management, utilizing different eco-friendly, non-chemical methods, represents a strategy for controlling postharvest decay and reducing economic losses in horticultural crops that is being actively developed and commercialized. In the present study, the use of the biocontrol yeast Pichia kluyveri combined with UV-C treatment was evaluated for its ability to control postharvest decay of king oyster mushrooms (Plerrotus eryngii) caused by Lactococcus lactis subsp. lactis. As stand-alone treatments, the use of P. kluyveri or UV-C significantly reduced disease development, relative to untreated mushrooms. The control effects on L. lactis decay and natural infection were significantly improved by the combination of P. kluyveri and UV-C, compared to either treatment alone. Treatment of mushrooms with P. kluyveri or UV-C alone and in combination also induced the activity the antioxidant enzymes of catalase and superoxide dismutase in treated mushrooms, and also enhanced the accumulation of antimicrobial substances, such as total phenolics and flavonoids. These data indicate that the mode of action by which UV-C enhances the biocontrol efficacy of P. kluyveri may be partially attributed to the elicitation of host defense responses in mushrooms. Integrating the use of a biocontrol agent with UV-C treatment represents an effective strategy for controlling postharvest decay in mushrooms.

中文翻译：

---

将生防酵母 Pichia kluyveri 与 UV-C 处理相结合，控制由乳酸乳球菌亚种引起的平菇（杏鲍菇）采后腐烂。乳酸菌

摘要 采后腐烂和衰老导致采收蘑菇的重大损失。综合管理利用不同的环保、非化学方法，代表了一种控制收获后腐烂和减少园艺作物经济损失的策略，目前正在积极开发和商业化。在本研究中，评估了生防酵母 Pichia kluyveri 结合 UV-C 处理的使用，以评估其控制由乳酸乳球菌亚种引起的牡蛎王菇（Plerrotus eryngii）采后腐烂的能力。乳酸。作为独立处理，相对于未经处理的蘑菇，使用 P. kluyveri 或 UV-C 显着减少了疾病的发展。P. kluyveri与UV-C联合使用显着提高了对乳酸乳球菌腐烂和自然感染的防治效果，与单独的任何一种治疗相比。单独和联合使用 P. kluyveri 或 UV-C 处理蘑菇也诱导了处理过的蘑菇中过氧化氢酶和超氧化物歧化酶的抗氧化酶的活性，并且还增强了抗微生物物质的积累，例如总酚类和黄酮类化合物。这些数据表明，UV-C 增强 P. kluyveri 生物防治功效的作用方式可能部分归因于蘑菇中宿主防御反应的引发。将生物防治剂的使用与 UV-C 处理相结合是控制蘑菇采后腐烂的有效策略。kluyveri 或 UV-C 单独和组合也诱导处理过的蘑菇中过氧化氢酶和超氧化物歧化酶的抗氧化酶的活性，并且还增强了抗菌物质的积累，例如总酚类和黄酮类化合物。这些数据表明，UV-C 增强 P. kluyveri 生物防治功效的作用方式可能部分归因于蘑菇中宿主防御反应的引发。将生物防治剂的使用与 UV-C 处理相结合是控制蘑菇采后腐烂的有效策略。kluyveri 或 UV-C 单独和组合也诱导处理过的蘑菇中过氧化氢酶和超氧化物歧化酶的抗氧化酶的活性，并且还增强了抗菌物质的积累，例如总酚类和黄酮类化合物。这些数据表明，UV-C 增强 P. kluyveri 生物防治功效的作用方式可能部分归因于蘑菇中宿主防御反应的引发。将生物防治剂的使用与 UV-C 处理相结合是控制蘑菇采后腐烂的有效策略。kluyveri 可能部分归因于在蘑菇中引发宿主防御反应。将生物防治剂的使用与 UV-C 处理相结合是控制蘑菇采后腐烂的有效策略。kluyveri 可能部分归因于在蘑菇中引发宿主防御反应。将生物防治剂的使用与 UV-C 处理相结合是控制蘑菇采后腐烂的有效策略。