Methanothermobacter thermautotrophicus ΔH (MTH) is a thermophilic hydrogenotrophic methanogenic archaeon capable of reducing CO₂ with H₂ to produce methane gas. It is the potential candidate in the biomethanation of CO₂ and CO in anaerobic reactors and biogas upgrading process. However, systematic studies addressing its genome conservation and function remain scant in this genome. In this study, we have evaluated its evolutionary resemblance and metabolic discrepancy, particularly in starvation survival systems by comparing the genomic contexts with Methanothermobacter marburgensis str. Marburg (MMG) and Methanobacterium formicicum DSM 1535 (MFO). The phylogenomic analysis of this study indicated that there was a strong phylogenomic signal among MTH, MMG, and MFO in the whole-genome tree. DNA replication machinery was conserved in the MTH genome and might have evolved at different evolution rates. Genome synteny analysis observed collinearity of either gene orders or gene families has to be maintained with syntenic blocks located in the syntenic out-paralogs. A genome-wide metabolic analysis identified some unique putative metabolic subsystems in MTH, which are proposed to determine its growth characteristics in diverse environments. MTH genome comprised of 93 unique genes-coding for starvation survival and stress-response proteins. These proteins confer its adaptation to nutritional deprivation and other abiotic stresses. MTH has a typical system to withstand its growth and cell viability during stable operation and recovery after prolonged starvation. Thus, the present work will provide an insight to improve the genome refinement and metabolic reconstruction in parallel to other closely related species.

Methanothermobacter thermautotrophicus ΔH（MTH）能够降低CO的嗜热产甲烷氢营养古细菌₂用H ₂以产生甲烷气体。它是厌氧反应器中CO ₂和CO的生物甲烷化和沼气提质工艺的潜在候选者。但是，有关该基因组保守性和功能的系统研究在该基因组中仍然很少。在这项研究中，我们通过将基因组背景与马氏甲烷温热杆菌的基因组环境进行了比较，评估了其进化相似性和代谢差异，特别是在饥饿生存系统中。马尔堡（MMG）和福建甲烷杆菌DSM 1535（MFO）。这项研究的系统进化分析表明，在全基因组树中，MTH，MMG和MFO之间存在很强的系统进化信号。DNA复制机制在MTH基因组中是保守的，并且可能以不同的进化速率进化。基因组同源性分析观察到，必须使用位于同系外旁系同源物中的同构区块来维持基因顺序或基因家族的共线性。全基因组代谢分析确定了MTH中一些独特的推定代谢子系统，这些分子被提出来确定其在不同环境中的生长特征。MTH基因组由93个独特的基因组成，这些基因编码饥饿生存和应激反应蛋白。这些蛋白质赋予其适应营养剥夺和其他非生物胁迫的能力。MTH具有典型的系统，可在长期饥饿后稳定运行和恢复期间承受其生长和细胞活力。因此，本工作将提供与其他紧密相关物种并行改善基因组细化和代谢重建的见识。