This study aimed to explore the role of miR-146b-3p in acute respiratory distress syndrome in septic mice. Ten mice were randomly selected as normal group (n = 10, without any treatment) and 60 septic mice with acute respiratory distress syndrome were divided into model group (n = 10, without any treatment), negative control (NC) mimic group (n = 10, injected with NC mimic), miR-146b-3p mimic group (n = 10, injected with miR-146b-3p mimic), si-NC group (n = 10, injected with PI3Kγ siRNA NC), si-PI3Kγ group (n = 10, injected with PI3Kγ silencing plasmid), and miR-146b-3p mimic + oe-PI3Kγ group (n = 10, injected with miR-146b-3p mimic + PI3Kγ overexpression plasmid). We found that miR-146b-3p negatively regulated PI3Kγ. Compared with normal group, model mice had decreased expression of miR-146b-3p, increased expressions of PI3Kγ, p-AKT, ASC, NLRP3 and Caspase-1 proteins, higher W/D ratio, and more serum IL-1β and IL-18 content (all P < 0.05). All indicators in miR-146b-3p mimic group and si-PI3Kγ group were significantly improved as compared to model group (all P < 0.05). Over-expression of PI3Kγ could weaken the treatment effect of miR-146b-3p mimic in model mice. Therefore, up-regulation of miR-146b-3p can inhibit PI3K/AKT signaling pathway to improve acute respiratory distress syndrome in septic mice.

这项研究旨在探讨miR-146b-3p在败血症小鼠急性呼吸窘迫综合征中的作用。随机选择十只小鼠作为正常组（n = 10，未进行任何治疗），并将60例急性呼吸窘迫综合征脓毒症小鼠分为模型组（n = 10，未进行任何治疗），阴性对照组（NC）模拟组（n = 10，注射NC模拟物），miR-146b-3p模拟物组（n = 10，注射miR-146b-3p模拟物），si-NC组（n = 10，注射PI3KγsiRNA NC），si-PI3Kγ组（n = 10，注射了PI3Kγ沉默质粒），而miR-146b-3p模拟物+oe-PI3Kγ组（n = 10，注射了miR-146b-3p模拟物+PI3Kγ过表达质粒）。我们发现miR-146b-3p负调控PI3Kγ。与正常组相比，模型小鼠的miR-146b-3p表达降低，PI3Kγ，p-AKT，ASC，MAPK表达增加。P  <0.05）。与模型组相比，miR-146b-3p模拟组和si-PI3Kγ组的所有指标均显着改善（所有P  <0.05）。PI3Kγ的过表达可能减弱miR-146b-3p模拟物对模型小鼠的治疗作用。因此，miR-146b-3p的上调可以抑制PI3K / AKT信号通路，以改善败血症小鼠的急性呼吸窘迫综合征。