A screening method using 15% CO₂ (v/v) as screening stress and a spotting plate method was developed to isolate microalgae with the potential to convert flue gas CO₂ to biomass. A total of six microalgal strains, belonging to the genera Chlorella, Heynigia, Desmodesmus, and Scenedesmus, were isolated from ponds near metallurgical/cement/power plants. The growth of these isolated strains was dramatically promoted at 5 to 15% CO₂ when they were cultivated in bubble column photobioreactors aerating with 0.03%, 5%, 10%, and 15% CO₂. The growth of Heynigia riparia SX01 in particular showed substantial improvement with the increase of CO₂ concentrations from 5 to 15%. Furthermore, the maximum biomass, overall biomass productivity, maximum biomass productivity, and maximum CO₂ fixation rate of these microalgal strains greatly increased at 5 to 15% CO₂ as well. Chlorella sorokiniana GS03 showed the highest values in maximum biomass productivity (0.36 g L⁻¹ day⁻¹) and maximum CO₂ fixation rate (0.66 g L⁻¹ day⁻¹) at 5% CO₂. Heynigia riparia SX01 exhibited the highest values of maximum biomass (3.28 g L⁻¹), overall biomass productivity (0.27 g L⁻¹ day⁻¹), maximum biomass productivity (0.39 g L⁻¹ day⁻¹), and maximum CO₂ fixation rate (0.71 g L⁻¹ day⁻¹) at 15% CO₂. This study provides not only an efficient screening method obtaining microalgae with wide CO₂ tolerance but also microalgal strains utilizing high levels of CO₂ up to 15% to produce biomass, which contributes to further exploration in converting real flue gas CO₂ into biomass feedstock.

开发了一种使用15％CO ₂（v / v）作为筛选应力的筛选方法和点样板法，以分离具有将烟气CO ₂转化为生物质的潜力的微藻。总共6个微藻株，属于下列属小球藻，Heynigia，链带藻，和栅藻，从邻近的冶金/水泥/发电厂池塘隔离。这些分离的菌株的生长在5至15％急剧促进CO ₂，当他们在气泡塔型光生物反应器与0.03％，5％，10％，和15％CO充气培养₂。河豚草的生长特别地，随着CO ₂浓度从5％增加到15％，SX01表现出显着改善。此外，这些微藻菌株的最大生物量，总生物量生产率，最大生物量生产率和最大CO ₂固定率也以5％至15％的CO ₂大大增加。Sorokiniana小球藻GS03在5％CO _2下的最大生物量生产力（0.36 g L ^-1  day ^-1）和最大CO ₂固定率（0.66 g L ^-1  day ^-1）最高。阔叶禾本科（Heynigia riparia） SX01表现出最大生物量的最大值（3.28 g L ^-1），总生物量生产力（0.27 g L ^-1 天^-1），最大生物量生产力（0.39 g L ^-1 天^-1）和最大CO ₂固定率（0.71 g L ^-1 天^-1）在15％CO ₂。这项研究不仅提供了一种获得具有宽广的CO ₂耐受性的微藻的有效筛选方法，而且还提供了利用高达15％的高水平CO ₂产生生物质的微藻菌株，这有助于进一步探索将真实的烟气CO ₂转化为生物质原料。