Emerging evidence demonstrates that lncRNAs participate in various developmental processes in plants via post-transcription regulation. However, few lncRNAs have been identified as regulators of tiller development in wheat (Triticum aestivum L.). In this study, high-throughput ribosomal depleted RNA sequencing was performed on the tillering nodes of two pairs of near-isogenic lines that differed only in the tillering trait. We identified 5399 lncRNA transcripts using bioinformational analyses. KEGG pathway analysis revealed 74 common differentially expressed lncRNAs substantially enriched in photosynthesis-related, phenylpropanoid biosynthesis, phosphatidylinositol signaling, brassinosteroid biosynthesis, zeatin biosynthesis, and carotenoid biosynthesis pathways. Detailed functional annotations of target genes were used to identify 27 tillering-associated lncRNAs. Among these, 10 were in photosynthesis-related pathways; 15 were in secondary metabolite pathways; and 8 were in plant hormone pathways, with 6 enriched in two kinds of pathways. These findings contribute to identifying tillering-associated lncRNAs in wheat and enable further investigation into the functions and roles of key candidate lncRNAs, and more experimental evidence was also needed if breeders wanted to utilize these candidate lncRNAs in wheat crop yield improvement in the future.

中文翻译：

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在两对近等基因系中鉴定参与小麦分till发育的lncRNA。

新兴证据表明，lncRNA通过转录后调控参与植物的各种发育过程。然而，几乎没有lncRNA被鉴定为小麦分till发育的调控因子（普通小麦）L.）。在这项研究中，对两对仅在分er性状上不同的近等基因系的分nodes节进行了高通量核糖体耗竭的RNA测序。我们使用生物信息分析鉴定了5399个lncRNA转录本。KEGG通路分析揭示了74个常见的差异表达的lncRNA，它们在光合作用，苯丙烷类生物合成，磷脂酰肌醇信号传导，油菜素类固醇生物合成，玉米素蛋白生物合成和类胡萝卜素生物合成通路中基本富集。靶基因的详细功能注释用于鉴定27个与分asso相关的lncRNA。其中有10个处于光合作用相关的途径。15个处于次生代谢途径中；植物激素途径中有8种，其中两种途径中有6种富集。