Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, it is often challenging to acquire sufficient fresh sperm to fertilize the eggs that are obtained. In this study, we evaluate the possibility to store spotted wolffish sperm by refrigeration. Spotted wolffish sperm has the particularity that is already motile on stripping, and currently it is not possible to immobilize and reactivate. Thus, sperm refrigeration protocols should focus in extending this motility period that usually lasts up to 2 days. In a first experiment, we evaluated the possibility that the motility period of the sperm was limited by contamination with urine. The urea concentration in the sperm obtained both by stripping (17.10 ± 1.98 mg/dL) and directly from the testis (12.59 ± 2.37 mg/dL) was similar (p > 0.05), which indicate that the sperm collection method used avoid contamination with urine. Afterwards, we tested the possibility that the sperm motility period was limited by energy stores. The ATP concentration (initial value 5.65 ± 0.86 nmol/10⁹ cells) remained stable (p = 0.099) during 30 h after sperm collection, and similar values (p = 0.329) were recorded at end of sperm storage in both diluted (3.88 ± 1.35 nmol/10⁹ cells) and undiluted samples (4.76 ± 1.08 nmol/10⁹). This indicates that the low intracellular ATP consumption, derived from the slow sperm motility, can probably be compensated rapidly enough by mitochondrial synthesis of ATP in the spotted wolffish sperm. In both experiments, diluted sperm kept higher percentage of motile cells during the storage time.

圈养的斑狼鱼Anarhichas 的轻微繁殖依赖于体外受精。然而，获得足够的新鲜精子来使所获得的卵子受精通常具有挑战性。在这项研究中，我们评估了通过冷藏储存斑狼鱼精子的可能性。斑狼鱼精子的特殊性是在剥离时就已经具有活动性，目前还无法固定和重新激活。因此，精子冷藏方案应侧重于延长通常持续长达 2 天的运动期。在第一个实验中，我们评估了精子的活动时间因尿液污染而受到限制的可能性。通过剥离获得的精子 (17.10 ± 1.98 mg/dL) 和直接从睾丸获得的精子 (12.59 ± 2.37 mg/dL) 中的尿素浓度相似 ( p > 0.05)，这表明所使用的精子采集方法避免了污染与尿液。随后，我们测试了精子活动时间受能量储存限制的可能性。精子采集后 30 小时内 ATP 浓度（初始值 5.65 ± 0.86 nmol/10 ^{9 个}细胞）保持稳定 ( p = 0.099)，并且在精子储存结束时在两种稀释液中记录了相似的值 ( p = 0.329) (3.88 ± 1.35 nmol/10 ^{9 个}细胞）和未稀释的样品（4.76 ± 1.08 nmol/10 ⁹ ）。这表明，由于精子运动缓慢而导致的细胞内 ATP 消耗量较低，可能可以通过斑狼鱼精子中线粒体合成 ATP 来足够快地补偿。在这两个实验中，稀释的精子在储存期间保留了较高比例的运动细胞。