Durum wheat × Imperata cylindrica hybridization was carried out utilizing two durum wheat genotypes as maternal parents (A-9-30-1 and PDW 314) and I. cylindrica as a pollen source followed by in vivo application of colchicine to establish a standard protocol for colchicine application aimed at enhancement of doubled haploid production in durum wheat. Various concentrations of colchicine ranging from 500 to 7000 ppm were injected to the pollinated spikes at different time interval viz., 24 h, 48 h and 72 h after pollination. Application of 5000–7000 ppm colchicine at 48 h after pollination was established as the optimum colchicine dose for chromosome doubling as confirmed through cytological studies in durum wheat using I. cylindrica-mediated chromosome elimination approach. PDW 314 was identified as a durum wheat genotype having more potential for doubled haploid induction via in vivo colchicine application than A-9-30-1. Identification of optimum dose of colchicine for application at in vivo level for induction of doubled haploids has opened new vistas in durum wheat improvement programme.

中文翻译：

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使用白茅草介导的染色体消除方法在体内操作秋水仙碱以提高硬粒小麦中 DH 的生产效率

使用两种硬粒小麦基因型作为母本（A-9-30-1 和 PDW 314）和 I. cylindrica 作为花粉来源，然后在体内应用秋水仙碱，进行硬粒小麦×白茅草杂交，以建立标准方案。秋水仙碱应用旨在提高硬粒小麦双单倍体产量。在不同的时间间隔，即授粉后 24 小时、48 小时和 72 小时，将 500 至 7000 ppm 的各种浓度的秋水仙碱注射到授粉穗中。使用 I. cylindrica 介导的染色体消除方法对硬粒小麦进行细胞学研究证实，授粉后 48 小时应用 5000–7000 ppm 秋水仙碱被确定为染色体加倍的最佳秋水仙碱剂量。PDW 314 被鉴定为一种硬粒小麦基因型，与 A-9-30-1 相比，通过体内秋水仙碱应用具有更大的双单倍体诱导潜力。确定在体内水平应用以诱导双单倍体的秋水仙碱最佳剂量为硬粒小麦改良计划开辟了新的前景。