We studied cytotoxic activity of new tetranitrosyl NO-generating binuclear iron—sulfur [Fe—S] complexes containing different ligands in the molecule against tumor cells in vitro. Cytotoxic activity of the most active complex with cysteamine (CysAm) was compared with that of antitumor drug cisplatin. Caspase activation and morphological changes in cells were visualized by fluorescence microscopy. Fluorescence of active caspases 3 and 7 and changes in nuclear DNA in cells in the presence of CyAm were detected by using fluorochrome-labeled inhibitor of caspases (FLICA) and Hoechst and propidium iodide reagents. Similar cytotoxic activities of CyAm and cisplatin were demonstrated in various human tumor cell lines of different histogenesis. Therefore, a new class of NO-donating [Fe—S] complexes can provide the base of potential drugs for chemotherapy with a new mechanism of action.

中文翻译：

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新型亚硝基铁硫配合物在体外人肿瘤细胞中细胞毒活性的比较分析

我们在体外研究了分子中含有不同配体的新型四亚硝基 NO 生成双核铁硫 [Fe-S] 复合物对肿瘤细胞的细胞毒活性。将最活跃的半胱胺复合物 (CysAm) 的细胞毒活性与抗肿瘤药物顺铂的细胞毒活性进行了比较。通过荧光显微镜观察细胞中的半胱天冬酶活化和形态变化。使用荧光染料标记的半胱天冬酶抑制剂 (FLICA) 和 Hoechst 和碘化丙啶试剂检测活性半胱天冬酶 3 和 7 的荧光以及在 CyAm 存在下细胞核 DNA 的变化。CyAm 和顺铂的类似细胞毒活性在不同组织发生的各种人类肿瘤细胞系中得到证实。所以，