Late leaf spot (LLS) and rust diseases are the major devastating diseases in groundnut that lowers crop yield and fodder quality. Marker assisted backcross breeding was employed to introgress LLS and rust diseases resistance QTL region from GPBD 4 into an India’s popular groundnut variety TMV 2. The MABC approach was exercised using a total of eight disease resistance-linked markers viz.., IPAHM103, GM1573, GM1009, Seq8D09, GM2009, GM1536, GM2301 and GM2079 present in the QTL region. After three backcrosses and selfing, a total of 155 BC3F2 lines were developed from the cross TMV 2 x GPBD 4. Ten BC3F2 homozygous introgression lines were identified with resistance QTL region and subjected to background analysis. A genome recovery upto 99.80% was observed among introgression lines and advanced to further generations for field evaluation. Finally, six ILs with enhanced late leaf spot and rust diseases resistance were identified. Those ILs had significantly increased pod yield (42–65%) over the recurrent parent TMV 2 with enhanced resistance to LLS and rust diseases. Multi-location field evaluation of these ILs could leads to the release of improved varieties and also can be used as genetic resources in the groundnut improvement.

中文翻译：

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标记辅助 QTL 区域渗入以提高优良和流行品种花生 (Arachis hypogaea L.) cv TMV 2 的晚叶斑病和锈病抗性

晚叶斑病 (LLS) 和锈病是花生的主要破坏性病害，会降低作物产量和饲料质量。采用标记辅助回交育种将 LLS 和锈病抗性 QTL 区域从 GPBD 4 渗入印度流行的花生品种 TMV 2。 MABC 方法使用总共 8 个抗病连锁标记，即 IPAHM103、GM1573、 GM1009、Seq8D09、GM2009、GM1536、GM2301 和 GM2079 存在于 QTL 区域。经过3次回交和自交，从TMV 2 x GPBD 4杂交共开发出155个BC3F2品系。鉴定了10个具有抗性QTL区域的BC3F2纯合基因渗入品系并进行背景分析。在基因渗入品系中观察到高达 99.80% 的基因组恢复率，并进一步进行田间评估。最后，鉴定出6个具有增强的晚叶斑病和锈病抗性的ILs。与轮回亲本 TMV 2 相比，这些 IL 显着增加了豆荚产量（42-65%），并增强了对 LLS 和锈病的抗性。对这些ILs进行多地点田间评价，可以促进良种的释放，也可以作为花生改良的遗传资源。