The current study was executed to explore the nutritive potential of low-cost agro-industrial waste substrates including anaerobically treated distillery spent wash (ADSW) and rice straw (RS) for enzyme production (cellulase and xylanase). The statistical model, Box–Behnken Design (BBD), was utilized to optimize the enzyme production through Aspergillus heteromorphus under liquid state bioconversion on examining the interactive interaction between substrate (ADSW), co-substrate (RS), temperature and initial pH. The BBD results revealed that the optimum levels of different factors for enzymes production were 6% ADSW, 3% RS, pH 5 and temperature 32.5 °C. The highest exoglucanase, xylanase and endoglucanase enzyme activities under optimum conditions were 6.3 IU/mL, 11.6 IU/mL and 8.1 IU/mL, respectively. The results confined that the agro-industrial waste substrate could be a cost-effective and eco-friendly alternative to produce value-added products like enzymes.

中文翻译：

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探索使用异形曲霉生产纤维素酶和木聚糖酶的低成本农用工业废料底物

进行当前的研究是为了探索低成本农业工业废料的营养潜力，包括厌氧处理的酿酒厂废洗液（ADSW）和稻草（RS）用于生产酶（纤维素酶和木聚糖酶）。统计模型Box–Behnken设计（BBD）用于优化异形曲霉的酶生产在液态生物转化中研究底物（ADSW），共底物（RS），温度和初始pH之间的相互作用。BBD结果显示，酶产生的不同因素的最佳水平为6％ADSW，3％RS，pH 5和温度32.5°C。在最佳条件下，最高的外切葡聚糖酶，木聚糖酶和内切葡聚糖酶的活性分别为6.3 IU / mL，11.6 IU / mL和8.1 IU / mL。结果表明，农用工业废弃物基质可以作为生产增值产品（如酶）的具有成本效益和生态友好的替代品。