Chemical methods for modification of proteins.,Organic & Biomolecular Chemistry

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Chemical methods for modification of proteins.
Organic & Biomolecular Chemistry ( IF 2.9 ) Pub Date : 2020-06-02 , DOI: 10.1039/d0ob00857e
Neelesh C Reddy ₁ , Mohan Kumar ₁ , Rajib Molla ₁ , Vishal Rai ₁

Affiliation

The library of chemical reactions for C–C and C–heteroatom bond formation is exceptional. The understanding of reactivity and diverse aspects of selectivity facilitates the functional group transformation of high complexity. However, the same is not valid for proteins as an organic substrate. Gratifyingly, we can translate some of the pre-existing reactions for developing methods for the modification of proteins. Also, there is enormous potential to create a new knowledge domain that will be unique to the densely functionalized architecture of proteins. At the outset, we outlined a few concepts that bridge the gap between chemical reactions with small molecules and proteins. Next, we introduced the key attributes and challenges associated with the selectivity that emerges due to the presence of multiple types and copies of functional groups. The examples with nucleophilic amino acids outline the chemoselectivity-associated features. Gradually, the discussion moves toward the concepts that led to the successful realization of site-selectivity and N-terminus residue-specificity. The attributes of organic chemistry that emerge due to the multifunctional organization of the substrate are marked. The last section overviews the analysis of protein bioconjugates by mass spectrometry. Also, the review outlines the unmet needs and opportunities.

中文翻译：

蛋白质修饰的化学方法。

C–C和C–杂原子键形成的化学反应库非常出色。对反应性和选择性的不同方面的理解促进了高度复杂的官能团转化。但是，对于蛋白质作为有机底物来说，这是无效的。令人欣慰的是，我们可以将一些先前存在的反应翻译为蛋白质修饰方法的开发。同样，具有巨大的潜力来创建一个新的知识域，该域对于蛋白质的密集功能化体系结构而言是唯一的。首先，我们概述了一些概念，这些概念弥合了化学反应与小分子和蛋白质之间的鸿沟。接下来，我们介绍了由于存在多种类型和功能基团副本而出现的与选择性相关的关键属性和挑战。具有亲核氨基酸的实例概述了化学选择性相关的特征。逐渐地，讨论转向了导致成功实现位点选择性和N末端残基特异性的概念。标记了由于基质的多功能组织而出现的有机化学属性。最后一部分概述了通过质谱分析蛋白质生物缀合物的方法。此外，审查还概述了未满足的需求和机会。最后一部分概述了通过质谱分析蛋白质生物缀合物的方法。此外，审查还概述了未满足的需求和机会。最后一部分概述了通过质谱分析蛋白质生物缀合物的方法。此外，审查还概述了未满足的需求和机会。

更新日期：2020-07-01

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