Wogonin (5,7-dihydroxy-8-methoxyflavone) is an ingredient of the extracts from Scutellaria baicalensis, which has documented a wide spectrum of anti-inflammatory and antitumor activities, including inhibiting regulatory T cells, regulating effector T cell functions, and mediating macrophage immunity. However, the potential effect of Wogonin on B cells has not been fully understood. Here, our results showed that Wogonin inhibited IL-10 secretion in B cells. When purified B cells were activated by lipopolysaccharide (LPS) in vitro, the amount of IL-10 production in supernatant was decreased by Wogonin significantly. The protective role of B cells on dextran sulfate sodium- (DSS-) induced colitis was alleviated after exposure to Wogonin. Furthermore, administration of Wogonin on LPS-treated B cells suppressed phosphorylation of STAT3 and ERK, but not AKT. Interestingly, among those IL-10 signaling-associated transcription factors, mRNA and protein levels of Hif-1α were specifically decreased by Wogonin. Overall, our study indicates that Wogonin suppresses potentially IL-10 production in B cells via inhibition of the STAT3 and ERK signaling pathway as well as inhibition of mRNA and protein levels of the transcription factor Hif-1α. These results provide novel and potential molecular targets of Wogonin in B cells and help us further understand its mechanism of action, which could potentially improve its clinical application in the future.

中文翻译：

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Wogonin通过STAT3和ERK信号通路抑制B细胞中IL-10的产生。

Wogonin（5,7-dihydroxy-8-methoxyflavone）是黄S提取物的成分，已证明其广泛的抗炎和抗肿瘤活性，包括抑制调节性T细胞，调节效应T细胞功能和介导巨噬细胞免疫力。但是，尚未完全了解Wogonin对B细胞的潜在作用。在这里，我们的结果表明，Wogonin抑制B细胞中的IL-10分泌。当纯化的B细胞在体外被脂多糖（LPS）激活时，Wogonin显着降低了上清液中IL-10的产生量。暴露于Wogonin后，B细胞对硫酸葡聚糖硫酸钠（DSS-）引起的结肠炎的保护作用减弱。此外，在经LPS处理的B细胞上服用Wogonin可抑制STAT3和ERK的磷酸化，但不是AKT。有趣的是，在那些与IL-10信号相关的转录因子中，Hif-1的mRNA和蛋白水平α被沃贡宁特别地降低。总体而言，我们的研究表明，Wogonin通过抑制STAT3和ERK信号通路以及抑制转录因子Hif- 1α的mRNA和蛋白质水平来抑制B细胞中IL-10的产生。这些结果为Wogonin在B细胞中提供了新的和潜在的分子靶标，并帮助我们进一步了解其作用机理，这可能在将来改善其临床应用。