Burn patients with multidrug-resistant Pseudomonas aeruginosa infections commonly suffer from high morbidity and mortality, which present a major challenge to healthcare systems throughout the world. Outer membrane protein F (OprF), as a main outer membrane porin, is required for full virulence expression of P. aeruginosa. The aim of this study was to evaluate the protective efficacy of egg yolk-specific antibody (IgY) raised against recombinant OprF (r-OprF) protein in a murine burn model of infection. The hens were immunized with r-OprF, and anti-r-OprF IgY was purified using salt precipitation. Groups of mice were injected with different regimens of anti-OprF IgY or control IgY (C-IgY). Infections were caused by subcutaneous injection of P. aeruginosa strain PAO1 at the burn site. Mice were monitored for mortality for 5 days. The functional activity of anti-OprF IgY was determined by in vitro invasion assays. Immunotherapy with anti-OprF IgY resulted in a significant improvement in the survival of mice infected by P. aeruginosa from 25% to 87.5% compared with the C-IgY and PBS. The anti-OprF IgY decreased the invasion of P. aeruginosa PAO1 into the A549. Passive immunization with anti-OprF IgY led to an efficacious protection against P. aeruginosa burn infection in the burn model.

中文翻译：

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带有针对外部膜蛋白F的IgY抗体的免疫疗法可保护烧伤的小鼠免受铜绿假单胞菌感染。

具有多重耐药性的铜绿假单胞菌感染的烧伤患者通常患有高发病率和高死亡率，这对全世界的医疗系统提出了重大挑战。铜绿假单胞菌的完全毒力表达需要外膜蛋白F（OprF）作为主要的外膜孔蛋白。这项研究的目的是评估在小鼠烧伤感染模型中针对重组OprF（r-OprF）蛋白产生的蛋黄特异性抗体（IgY）的保护作用。用r-OprF免疫母鸡，并通过盐沉淀纯化抗r-OprF IgY。给小鼠组注射不同方案的抗OprF IgY或对照IgY（C-IgY）。皮下注射铜绿假单胞菌引起感染烧伤部位的PAO1菌株。监测小鼠的死亡率5天。抗OprF IgY的功能活性通过体外侵袭测定法确定。与C-IgY和PBS相比，抗OprF IgY的免疫疗法可将铜绿假单胞菌感染的小鼠的存活率从25％显着提高至87.5％。抗OprF IgY减少了铜绿假单胞菌PAO1侵入A549。抗OprF IgY的被动免疫可在烧伤模型中有效抵抗铜绿假单胞菌烧伤感染。