We described a sacrificial molding for the formation of microfluidic networks. In this molding, the micromolded calcium alginate (Ca-Alg) is introduced as a sacrificial template. The basis of this procedure is fabricating a micromolded Ca-Alg hydrogel and encapsulating this model within a second gel and removing it by ion-exchange to leave a microchannel in the remaining gel. This microfluidic system can readily deliver solutes into the channels and even control the transport of solutes from channels into the bulk of the gels. Furthermore, the perfused vascular channels can sustain the metabolic activity of encapsulated cells, indicating the feasibility of this microfluidic system in the field of tissue engineering.

中文翻译：

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使用微模制海藻酸盐凝胶作为组织工程牺牲材料的微流体系统的制造

我们描述了用于形成微流体网络的牺牲成型。在这种成型中，微成型的海藻酸钙 (Ca-Alg) 被引入作为牺牲模板。此过程的基础是制造微成型 Ca-Alg 水凝胶，并将该模型封装在第二个凝胶中，并通过离子交换将其移除，从而在剩余的凝胶中留下微通道。这种微流体系统可以很容易地将溶质输送到通道中，甚至可以控制溶质从通道到大部分凝胶的运输。此外，灌注的血管通道可以维持封装细胞的代谢活动，表明这种微流体系统在组织工程领域的可行性。