Background. The association of miR-28-5p with colon cancer remains to be elucidated. This study aimed to determine the clinical significance and prognostic value of miR-28-5p in colon cancer. Methods. We retrospectively analyzed the data of miR-28-5p in colon adenocarcinoma data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), and the data was divided into cancer group and normal group, respectively. Forty colon cancer tissues and adjacent normal tissues were collected and tested by qRT-PCR methods. The difference of the miR-28-5p expression between colon cancer and normal tissues was compared. The clinical significance of miR-28-5p in colon cancer and the association with the survival were determined. The predictive value of miR-28-5p in clinical features was determined using receiver operating characteristic curve. The target genes of miR-28-5p were identified, and the functional of target genes was performed using bioinformatics analysis. Results: The expression of miR-28-5p was increased in colon cancer tissues compared with normal controls (). The expression of miR-28-5p was significantly increased in tissues with distant metastases compared with that without distant metastases (). Patients with high expression of miR-28-5p have a shorter survival time than those with low expression (). Cox analysis showed that miR-28-5p was an independent predictor for the survival of patients (). Combination of miR-28-5p with TNM stage and clinical stage can improve the prognostic value for the patients (). miR-28-5p has a moderate predictive value in predicting the TNM stage and clinical stage (T stage: ; N stage: , M stage: ; clinical stage: ). 711 potential target genes of miR-28-5p were screened; their function and pathways were identified. Conclusions: This study demonstrated that miR-28-5p was increased in colon cancer and can be an independent indicator for the overall survival in patients with colon cancer.

中文翻译：

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miR-28-5p 在结肠癌中的临床意义和预后价值。

背景。miR-28-5p 与结肠癌的关系仍有待阐明。本研究旨在确定 miR-28-5p 在结肠癌中的临床意义和预后价值。方法. 我们回顾性分析了癌症基因组图谱（TCGA）和基因表达综合（GEO）的结肠​​腺癌数据中miR-28-5p的数据，并将数据分别分为癌症组和正常组。收集四十个结肠癌组织和邻近的正常组织并通过qRT-PCR方法进行检测。比较结肠癌组织与正常组织间miR-28-5p表达的差异。确定了 miR-28-5p 在结肠癌中的临床意义以及与生存的关系。使用受试者工作特征曲线确定 miR-28-5p 在临床特征中的预测价值。鉴定了miR-28-5p的靶基因，并使用生物信息学分析进行靶基因的功能。结果：与正常对照相比，结肠癌组织中miR-28-5p的表达增加（）。与没有远处转移的组织相比，有远处转移的组织中 miR-28-5p 的表达显着增加。）。miR-28-5p高表达的患者比低表达的患者存活时间更短。）。Cox 分析显示 miR-28-5p 是患者生存的独立预测因子。）。miR-28-5p与TNM分期和临床分期相结合可以提高患者的预后价值。）。miR-28-5p在预测TNM分期和临床分期（T分期：; N阶段：， M阶段：; 临床阶段：）。筛选出711个miR-28-5p的潜在靶基因；确定了它们的功能和途径。结论：本研究表明 miR-28-5p 在结肠癌中增加，可以作为结肠癌患者总生存期的独立指标。