The ability of mass spectrometry for discrimination between protein and peptide masses which are unique to specific pathogens provides an accurate and fast method for the detection of different types of pathogens, especially viruses. Capsid proteins are specific to each virus and can be used as a biomarker for detection of this pathogen. On the other hand, single-chain variable fragment (scFv) antibodies have been recently used to enhance the accuracy of immunoassay techniques. So conjugation of mass spectrometry and scFv antibody provides a very accurate and fast method for the detection of viruses. In this report, for the first time, we have immobilized scFv antibody of fig mosaic virus (FMV) on the magnetic nanoparticles (MNPs) to extract the virus capsid protein from complex biological media and subsequently identified this protein through both its intact molecular mass and peptide mass fingerprint (PMF) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).

中文翻译：

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单链可变片段抗体与磁性纳米粒子的结合以及无花叶病毒的MALDI TOF质谱筛选。

质谱法可区分特定病原体特有的蛋白质和多肽质量的能力，为检测不同类型的病原体（尤其是病毒）提供了一种准确而快速的方法。衣壳蛋白对每种病毒都有特异性，可用作检测该病原体的生物标记。另一方面，最近已使用单链可变片段（scFv）抗体来增强免疫测定技术的准确性。因此，质谱与scFv抗体的结合提供了一种非常准确，快速的病毒检测方法。在这份报告中，这是第一次